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Yes, urine will kill moss, especially on hard surfaces like driveways where I am using it. It may take a few applications, (I just pour it out from a container directly on top of the moss, making sure that the moss is saturated; do this on a dry day so it absorbs well), but it will kill it, and then time will remove the actual moss material by water erosion, wind, other people and animals traveling over it, etc.

The best part after it is finally eroded away is that it will take a long long time to return since that surface has now been "treated" and will not support new moss growth, (the same effect occured when I accidentally spilled some gas from a lawn mower on the edge of the driveway over 15 years ago, and that small area is still moss free).

Note that if it is applied when wet or raining it will get too diluted to be effective and will actually act as a fertizler.

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Q: Will urine kill moss
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