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We use internal standard for the identification of that compound which we want to know the concentration.

No effect of the injection volume of sample.

But Now a days Auto injector is coming very good quality, so we can control the injection volume of sample.

So we do not need any internal standard.

Nikhil

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Q: Why internal standard use in GC?
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Why is it necessary to use an internal standard in the GLC assay?

Internal standard is primarily used to increase the accuracy and precision of analytical methods that have large inherent variability. The method is used in chromatography (GC, HPLC) where a compound similar to the analyte of interest is added to the sample and run. By having the analyte and the standard elute in the same run, the run to run variability is eliminated giving more precise results. Obviously one needs to calibrate the responses of the internal standard with that of the analyte. Incidental benefits are saving time and money by having less runs. Hope this is useful. Jay, Winnipeg, Canada


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