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In the episode 'The Courtship of Stewie's Father' (UK Season 5/US Season 4), Brian sings it to Peter in an attempt to cheer him up, but the orginial 'It's Peanut Butter Jelly Time' was sung by a dancing banana. You can watch the original flash cartoon version here: http://www.albinoblacksheep.com/flash/banana.php. You can watch the Family Guy version on YouTube by typing 'family guy peanut butter jelly time'.
then when stewie was traveling back in time. stewie went back in time where brian sang the banana song. then sang it t peter instead of brian.
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BeauPeanut Butter Jelly Time (PBJT) is a flash animation that consists of an animated Dancing Banana character and the song "Peanut Butter Jelly Time" by The Buckwheat Boyz. Known for being both obnoxious and funny, the Dancing Banana video became a hit in the early 2000s, leading to PBJT-related merchandise and dozens of parodies and tribute videos on YouTube.
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