Joseph Poole, better known as Wednesday 13, has never publicly discussed his sexual orientation. What we can say is that he was married to a woman and has one child.
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His openly gay and a member of the Wednesday club, recruited by Captain Wednesday himself.
Nothing is known of her sexual orientation.
Soko's sexual orientation is heterosexual/straight.
The Joker's sexual orientation is not written into his character.
The fictional character of Yoshi is not depicted with sexual orientation.