Jello Biafra was married to female Therese Soder, and also dated Cynthia Plaster Caster. He has not made any other official announcements of his sexuality, therefore believed to be straight.
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Nothing is known of her sexual orientation.
The Joker's sexual orientation is not written into his character.
Soko's sexual orientation is heterosexual/straight.
The fictional character of Yoshi is not depicted with sexual orientation.
Frankie Muniz has not stated his sexual orientation.