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Im guessing it was an oboe could not tell? The original instrument was a cello that was tuned specially for the music for Noir.
It can mean a couple of different things. In solfege, the art of singing without the aid of a tuned instrument, the syllables do re mi, etc can take the place of the notes of a song, with 'do' representing the 'tonic', no matter what the key signature is. This is the moveable do system. Do can also be assigned to the standard pitch C, with the other syllables following accordingly. So mi can be the note a major third up from a song's tonic or key, and mi can also represent the fixed tone E. In most uses in the US, the moveable do is used.
I believe it was an open E tuning.
All of the strings are tuned down a half step, with the low E string tuned down a step and a half. It would be (from lowest to highest) C#, G#, C#, F#, A#, and D#.
True