She isn't gay. She pretended to be a lesbian in the episode Brian Sings and Swings but she's liked & had sex with so many guys, it's rather apparent she is not gay.
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The episode where Stewie finds out what being gay is is "Chick Cancer" Volume 5 episode 8.
yes he is
Stewart "Stewie" Gilligan Griffin, at a year old, he is the youngest son of Peter & Lois Griffin.He's an infant genius who dabbles in world domination and matricide. He's possibly bi-sexual, although it all depends on the jokes in the episode. If they want him to be attracted to girls for an episode he is, if the writers want him to be a little gay for an episode, than he is.
Shes not gay to begin with but in a later episode when they show stewie and meg grown up, meg has had a sex change and is called Ron. Though it never says shes gay full out.
Duo Maxwell encounters a gay couple on the battleship Libra.
After a little review on Google, i could not find any real proof of him being "gay". But, i did find a "tonight show" interview where he declared that was in horror after reading his gay parts in "Taking Woodstock". So, i would assume, no, he is not gay.