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Why pigment travel shorter than other pigments in chromatography?

Pigments travel at different rates in chromatography because of differences in their molecular size, polarity, and solubility in the solvent. Smaller, less polar pigments will travel further up the chromatography paper because they are less attracted to the stationary phase and can move more easily with the mobile phase.


What are the relations with pigment and chromatography?

In chromatography, pigments can be separated based on their differing affinities for the mobile and stationary phases. The different pigments will travel at different rates through the chromatography system, allowing for their separation and identification based on their unique colors and positions within the chromatogram. Pigments play a key role in chromatography as they provide a visible representation of the separation process.


How do pigments migrate?

Pigments migrate through a process called chromatography, where they are separated based on their size and solubility in a solvent. As the solvent travels up a chromatography paper, pigments with higher solubility move faster and travel further, resulting in distinct bands of separated pigments. The migration of pigments in chromatography is based on their individual chemical properties and interactions with the solvent.


Why does carotene travel farthest in chromatography of leaf pigments?

Carotene travels the farthest in chromatography of leaf pigments because it is the least soluble in the chromatography solvent. This means it interacts less with the solvent and more with the chromatography paper, allowing it to move further up the paper before the solvent front stops it.


What chemicals are used for separation of leaf pigments in paper chromatography?

In paper chromatography for separating leaf pigments, common solvents used include a mixture of organic solvents such as ethanol, acetone, or petroleum ether, often combined with water. These solvents help dissolve the pigments, allowing them to travel at different rates along the chromatography paper based on their solubility and affinity for the paper. As the solvent moves up the paper, pigments like chlorophyll, carotenoids, and anthocyanins separate into distinct bands.


How is chromatography used to study plant pigment?

chromatography is basically a technique used for the separation of different components.... plant pigment consist of different components..... the sample is taken nd spotted over a chromatography paper..... nd den it is kept in suitable solvent to get separated...... different components travel different distance on chromatogram.... dis is how v cn use chromatography to study plant pigments...


How do you separate a mixture of pigments from an ink cartage?

To separate a mixture of pigments from an ink cartridge, you can use chromatography, a technique that exploits the different solubilities of the pigments. Start by dissolving the ink in a suitable solvent, then apply a small spot of the solution onto chromatography paper. When the paper is placed in a solvent (like water or alcohol), different pigments will travel at different rates, allowing them to be separated. After the solvent has moved up the paper, you can analyze the distinct spots corresponding to various pigments.


How do pigments separate?

Pigments separate through a process called chromatography, where a mixture of pigments is dissolved in a solvent and then applied to a stationary phase, such as paper or a gel. As the solvent moves up the stationary phase, different pigments travel at different rates due to their varying affinities for the solvent and the stationary phase. This differential migration results in the separation of pigments, allowing them to be visualized as distinct spots or bands. Factors like solubility, size, and interactions with the medium influence the separation efficiency.


Why did the separation of pigments in the spinach extract occur as it did?

The separation of pigments in the spinach extract occurred due to differences in their solubility and affinity for the chromatography medium. Each pigment, such as chlorophyll a, chlorophyll b, and carotenoids, interacts uniquely with the solvent and the stationary phase, causing them to travel at different rates. As the solvent moves up the chromatography paper, the pigments are carried along but separate based on these varying interactions, resulting in distinct bands of color.


Why do different inks separate while using chromatography?

Different inks separate during chromatography because they contain different pigments with varying polarities. As the solvent moves up the paper, the pigments in the ink interact differently with the paper and solvent, causing some pigments to travel faster and further than others. This separation allows the individual pigments in the ink mixture to be identified by their distinct colors and positions on the chromatogram.


How can you explain the presence of the colors on the chromatogram that are absent in the original leaf?

The presence of colors on the chromatogram that are absent in the original leaf can be attributed to the separation of various pigments during the chromatography process. When the leaf extract is applied to the chromatography medium, different pigments travel at different rates based on their solubility and affinity for the stationary phase. This separation reveals pigments that may not be visually dominant in the leaf sample but are still present in smaller quantities. Additionally, some pigments may be masked by others in the original leaf, becoming visible only after separation.


Why can the pigment spot on chromatography paper not be submerged in solvent?

The pigment spot on chromatography paper should not be submerged in solvent because it will result in spreading and mixing of the pigments, making it difficult to distinguish them. Instead, the solvent should be allowed to slowly travel up the paper by capillary action, separating the pigments based on their solubility and mobility.