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In genetic engineering what takes up plasmid?
In genetic engineering, the bacterial cell takes up the plasmid
Are plasmid vectors helpful?
Plasmid vectors are an invaluable genetic engineering tool for inserting recombinant DNA sequences into different organisms or cells in culture.Plasmids are essentially circular DNA constructs composed of some essential elements like:An origin of replicationA multiple cloning site which consists of restriction sites where the recombinant DNA can be insertedMarker genes (like antibiotic resistance)reporter genes to confirm a successful transformation
How does the ti plasmid make genetic engineering plants a possibility?
It is capable of introducing exogenous genes into plant genomes. T-DNA genes are removed from the Ti plasmid and are replaced with the gene of interest.
How could you use genetic engineering techniques to make transformed bacteria that produce the enzyme?
Extract DNA from the cells of people who can make the digestion enzyme. Cut the DNA with restriction enzymes to cut out the gene that codes for the enzyme. Use gel electrophoresis to locate the gene. Then, use polymerase chain reaction to make copies of the gene. Choose a plasmid that has an antibiotic-resistance genetic marker, and cut the plasmid with the smae restriction enzyme use to cut out the hyman gene. Insert the copies of the human gene into the plasmids. Allow bacterial cells to take in the plasmids. Select for transformed bacteria by growing them in a culture containing the antibiotic. These bacteria will make the digestion enzyme.
A eukaryote used in genetic engineering is?
Virus and plasmid. Both can insert the target gene into the host's genome.
What is the role of the backbone plasmid in genetic engineering processes?
The backbone plasmid serves as a vehicle for carrying and replicating foreign DNA in genetic engineering processes. It provides the necessary elements for DNA replication, such as an origin of replication and antibiotic resistance genes, allowing the foreign DNA to be maintained and expressed in the host organism.
In genetic engineering what takes up plasmid?
In genetic engineering, the bacterial cell takes up the plasmid
True or false cells containing a plasmid with an antibiotic resistance gene will die in the presence of the antibiotic?
False. Cells containing a plasmid with an antibiotic resistance gene will survive in the presence of the antibiotic because they can produce the protein that confers resistance, allowing them to withstand the antibiotic's effects.
What structures contains genes for enzymes and antibiotic resistance?
plasmid
What type of plasmid is the source of the problem with antibiotic resistance?
The source of antibiotic resistance is often plasmids known as R plasmids, which carry genes that confer resistance to antibiotics. These plasmids can be transferred between bacteria, spreading antibiotic resistance throughout microbial populations.
Is it true cells containing a plasmid with an antibiotic resistance gene will die in the presence of the antibiotic?
false
A bacterial cell that has taken up plasmid DNA is?
called a transformed bacterial cell. The plasmid DNA can confer specific traits to the bacterial cell, such as antibiotic resistance, ability to produce certain proteins or enzymes, or other desired characteristics. This process is commonly used in genetic engineering and biotechnology research.
What type of gene is used to distinguish bacteria that carry a plasmid containing foreign DNA from those that don' t?
The plasmid that contains foreign DNA is engineered to also carry an antibiotic resistance gene. This antibiotic resistance gene codes for a protein that is able to inactivate an antibiotic thus keeping the cell alive. In the absence of the antibiotic resistance gene, the cells would not survive when exposed to an antibiotic. After transfection (the process of inserting the plasmid carrying the foreign gene into cells), the cells are gown in media containing an antibiotic. Cells that contain the plasmid (and therefore contain the antibiotic resistance gene) are able to survive in this medium. Cells that do not contain the plasmid (and therefore lack the antibiotic resistance gene) do not survive in this medium. The process described above is called selection
What is an extra loop of DNA that contains antibiotic resistance what gene?
An extra loop of DNA that carries antibiotic resistance genes is called a plasmid. These genes can provide bacteria with the ability to survive exposure to antibiotics.
How does plasmid allow for antibiotic resistance?
A plasmid (conjugative plasmid) that has a resistance gene on it, can transfer itself to another bacterial cell (called conjugation) or assist in the transfer of a non-conjugative plasmid that has a resistance gene to another cell (called mobilization). Whichever way it happens, once the plasmid is transfered to the new cell, this cell too may show signs of resistance to the particular antibiotic. This is one of the ways of the speard of resistance amongst bacteria
How will you identify cells carrying the recombinant plasmid?
Cells carrying the recombinant plasmid can be identified using selection markers, such as antibiotic resistance genes, that are present on the plasmid. By exposing the cells to the corresponding antibiotic, only those with the recombinant plasmid will survive. Additionally, molecular techniques like PCR or restriction enzyme digestion can be used to confirm the presence of the desired DNA sequence in the cells.
Why are plasmids pieces of DNA?
Plasmids are small, circular pieces of DNA separate from the chromosomal DNA in bacteria. They typically carry non-essential genes that provide certain advantages like antibiotic resistance or the ability to metabolize unusual substances. Their structure as separate genetic elements allows for rapid transfer between bacteria and adaptation to changing environmental conditions.
