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The outer lumen of the mitochondria. All those protons being pumped into the outer lumen make it quite acidic.

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The mitochondrial intermembrane space becomes acidic during mitochondrial electron transport due to the pumping of protons from the matrix across the inner membrane to the intermembrane space by complexes I, III, and IV of the electron transport chain. This forms an electrochemical gradient used to generate ATP through ATP synthase.

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10mo ago
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Q: What cellular compartment becomes acidic during mitochondrial electron transport?
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