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The three parts of a thermal cycling reaction are:

  1. Denaturation - takes place at a high temperature of around 94 - 96 degrees C. In this step the DNA double strands are taken apart with heat
  2. Annealing - the primers (forward and reverse) anneal or attach to sequence specific regions on the template DNA strands that were just denatured in the previous step
  3. Extension - DNA polymerase binds to the place where primer is bound and extends the strands in the 3' - 5' direction.
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