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What are the precautions to be taken while using UV spectrophotometer?
Wiki User
∙ 11y ago
One should not touch the optical assembly with bear hands. Optical surface of cuvettes should not be touched with fingers to avoid erratic reading.
Always ensure effectiveness of silica gel kept in instrument.
Preserve the instrument from dust particles.
Never wash the cuvettes with chromic acid.
Wash the cuvette with the distilled water and then with methanol before storage.
Check the calibration status before using the instrument and ensure the timely calibration.
Do not overfill the cuvettes.
Perform the visual inspection of cuvettes for suitability.
Wiki User
∙ 11y ago
- Wear appropriate personal protective equipment such as gloves and safety goggles.
- Ensure the instrument is calibrated and properly maintained.
- Use UV-transparent materials for sample holders to prevent absorption or interference.
- Avoid direct exposure to UV light and handle chemicals with care to prevent spills or exposure.
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