In theory yes but there's very little point in it as you can either get ethanol from crude oil through distilling and cracking through which you can also get diesel and petrol from. You can also make ethanol out of vegetables but again you can also make diesel. The main use of ethanol in running cars I would say is bio-petrol which is just a mixture of petrol and ethanol. All this achieves is less petrol being used. But yes, you can fuel your car on it after modifications but it's more commonly used as an additive to petrol.
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