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because giemsa stain is a mixture of methyl acetate Eosin and azure b. it doesnot contain any fixative that is why we use methanol to fix smear during giemsa stain
other stain like lieshman contain acetyl free methyl alcohol as a fixative so it does not need to fix slide stain with lieshman stain.
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During the performance of the simple staining procedure you failed to heat fix your EColi smear preparation Upon microscopic examination how would you expect this slide to differ from correct slide?
The bacterial smear will wash away during the staining procedure. This is avoided by heat fixation, during which the bacterial proteins are coagulated and fixed to the glass surface.
What is the function of methanol in the wright's stain solution?
to fixed the blood smear
What is gram variability?
If after staining, a smear reveals both purple(Gram +) and red cells(Gram -). This is generally an indication that the smear contains gram positive cells.
Give the reason for passing bacterial smear through the flame before staining?
It dries the smear and fixes the cells to the slide
What would you expect from a gram stain done on a slide that was heated too hot during the heat-fixed smear?
A Gram stain refers to a staining technique for the identification of bacteria. A Gram stain done on a slide that was heated too hot during the heat-fixed smear will destroy the cell wall of the bacteria.
What is the purpose of staining the smear in malachite green during spore staining?
the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.
During the performance of the simple staining procedure you failed to heat fix your EColi smear preparation Upon microscopic examination how would you expect this slide to differ from correct slide?
The bacterial smear will wash away during the staining procedure. This is avoided by heat fixation, during which the bacterial proteins are coagulated and fixed to the glass surface.
Why acetone free methanol in lieshmans stain?
methanol for fixing the smear and acetone free to avoid cell membrane lysis
What is the function of methanol in the wright's stain solution?
to fixed the blood smear
What is gram variability?
If after staining, a smear reveals both purple(Gram +) and red cells(Gram -). This is generally an indication that the smear contains gram positive cells.
Give the reason for passing bacterial smear through the flame before staining?
It dries the smear and fixes the cells to the slide
What would you expect from a gram stain done on a slide that was heated too hot during the heat-fixed smear?
A Gram stain refers to a staining technique for the identification of bacteria. A Gram stain done on a slide that was heated too hot during the heat-fixed smear will destroy the cell wall of the bacteria.
In gram staining why will very thick smears give inaccurate results?
If a smear exhibits uneven thickness, overlapping cells may not get the proper exposure to the reagents. This results in uneven or mottled staining. For example, in the thicker areas of the smear, gram-negative cells may not decolorize sufficiently and end up staining purple.
How would you define a properly prepared bacterial smear?
the bacteria are evenly spread out on the prepared slide in such a concentration that they are adequately separated from one another bacteria are not washed off the slide during staining bacterial form is not distorted
Is presence of grease or dirt on a glass slide going to result in poor smear preparation?
The smear will not adhere well if there is grease on the slide.
What happens if you don't heat fix a smear prior to staining of a bacterial slide?
smear will be washed( no smear will be left on the slide)
What is the purpose of smearing in microbiology?
Smear are made for preparing slides for staining which are used in microscopy. The main purpose of smear is to seprate cluster of microbial cells so that we can see them seprately which is helpfull in studying there morphology, and arrangement in colony
