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In Gram staining, some bacterial species will become Gram variable meaning they produce a false reaction for the following reasons: decolrized too long, too young/old of culture, stained too long. Also, time is important simply because you don't want to over or under stain. You won't be able to see your specimen as well.

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Loma Schowalter

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Q: Why is time an important factor in simple staining?
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