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During the procedures of a gram stain, decolorization is necessary to remove any stain or color from the gram negative cells. When a dye is used to stain gram positive cells, both gram positive and gram negative cells retain color. Mordant is used to bind the original stain to gram positive cells so when decolorizer is used they retain color. After the mordant has been used a decolorizer is used to wash away colo in gram negative cells. Counterstains are used to stain gram negative cells to better visualize contrasting cells. An example of a decolorizer that works well is ethanol.
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∙ 14y ago
Wiki User
∙ 10y agodecoulorising is performed to remove the methyl crystal violet stains
if the purple stain is removed, safranin staining performed after decoulorising will stain the cells pink. this means that the cell is gram negative
if the crystal violet stains are not removed by decolorising, it means that the cells are gram positive, because these cell have thicker cell walls hence acetone is not able to remove all the purple stains.
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Why is it important to include both gram negative and gram negative organisms in a smear prep when you are performing the gram stain?
The point of the gram stain is to differentiate between two things, with out both positive and negative gram cells there would be nothing to differentiate between, defeating the purpose of the process.
What is determined of the Gram Stain?
A gram stain is a cow
Difference between the gram stain ans acid fast stain reactions of E. coli?
Gram staining is useful in separating bacteria into two groups: Gram positive or Gram negative. They are separated into these groups based on their cell wall structure. Gram positive bacteria contain a thick layer of peptidoglycan in their cell walls, while Gram negative bacteria contain a very small layer of peptidoglycan (15% or less of what Gram positive cell walls contain). A primary stain is added, such as Crystal Violet, that will stain all of the bacteria. Then, a mordant (such as iodine and potassium iodide) is added to form a complex between the peptidoglycan and the stain, which will make the cell more resistant to decolorization. Then, a decolorizing agent is added, which will remove the primary stain from Gram negative bacteria, but will cause the cell walls in Gram positive bacteria to dehydrate, and therefore, they will retain the primary stain. Finally, a counterstain (typically safranin) is added to distinguish Gram positive from Gram negative. Gram positive cells will be purple, and Gram negative cells will be red if Crystal Violet and Safranin are used.Acid-fast staining is entirely different. Is is used to detect species of bacteria in the genera Mycobacteria and Nocardia. These bacteria are resistant to typical staining procedures, such as Gram staining, due to a thick, waxy lipid layer in the cell wall composed of mycolic acid. Heating of the bacteria with a very strong stain such as carbol-fuchsin is necessary to "melt" this lipid layer, and force the stain through the cell wall. Once the bacteria has cooled, they will be incredibly resistant to decolorization. Non-acid fast bacteria do not contain this mycolic acid layer, and therefore, they will decolorize much easier, and are then stained with a counterstain to distinguish Acid-Fast bacteria from Non-Acid-Fast bacteria.
Can a Gram stain a prion?
Protists are often stained using a silver stain, not a Gram stain.
What is gram stain of rotavirus?
gram -
Is B subtilis a gram positive or gram negative bacterium?
gram positive Exactly. When doing a gram stain on B. subtilis, this bacterium resists decolorization (keping the first stain and NOT taking on the color of the secondary stain). Therefore, this bacterium is gram (+).
What is the most crucial step in the gram stain procedure?
The most critical step of gram staining is the decolorization step as crystal violet stain will be removed from both G+ve & G-ve cells if the decolorizing agent(e.g alchohol ) is left on too long.
Why is it important to include both gram negative and gram negative organisms in a smear prep when you are performing the gram stain?
The point of the gram stain is to differentiate between two things, with out both positive and negative gram cells there would be nothing to differentiate between, defeating the purpose of the process.
What is determined of the Gram Stain?
A gram stain is a cow
Why do all cells stain purple in the flagella stain but not in the gram stain?
The gram stain uses a decolorizing product so it is possible to differentiate between the gram and the gram cells. Gram positive cells stain purple in color.
Difference between the gram stain ans acid fast stain reactions of E. coli?
Gram staining is useful in separating bacteria into two groups: Gram positive or Gram negative. They are separated into these groups based on their cell wall structure. Gram positive bacteria contain a thick layer of peptidoglycan in their cell walls, while Gram negative bacteria contain a very small layer of peptidoglycan (15% or less of what Gram positive cell walls contain). A primary stain is added, such as Crystal Violet, that will stain all of the bacteria. Then, a mordant (such as iodine and potassium iodide) is added to form a complex between the peptidoglycan and the stain, which will make the cell more resistant to decolorization. Then, a decolorizing agent is added, which will remove the primary stain from Gram negative bacteria, but will cause the cell walls in Gram positive bacteria to dehydrate, and therefore, they will retain the primary stain. Finally, a counterstain (typically safranin) is added to distinguish Gram positive from Gram negative. Gram positive cells will be purple, and Gram negative cells will be red if Crystal Violet and Safranin are used.Acid-fast staining is entirely different. Is is used to detect species of bacteria in the genera Mycobacteria and Nocardia. These bacteria are resistant to typical staining procedures, such as Gram staining, due to a thick, waxy lipid layer in the cell wall composed of mycolic acid. Heating of the bacteria with a very strong stain such as carbol-fuchsin is necessary to "melt" this lipid layer, and force the stain through the cell wall. Once the bacteria has cooled, they will be incredibly resistant to decolorization. Non-acid fast bacteria do not contain this mycolic acid layer, and therefore, they will decolorize much easier, and are then stained with a counterstain to distinguish Acid-Fast bacteria from Non-Acid-Fast bacteria.
Theories of principle of gram stain reactions?
Gram stain
What is the gram stain for cholera?
Gram Negative
Can a Gram stain a prion?
Protists are often stained using a silver stain, not a Gram stain.
What is the gram stain of H5N1?
The Gram stain is used for bacteria and not for viruses.
What is the Gram stain for measles?
Bacteria stain either gram-positive or gram-negative based on the presence or absence of a cell wall. Viruses do not pick up a gram stain.
What happens if you forget to add acetone to a gram negative stain?
When performing the Gram stain, acetone is used as a wash step between the iodine and safranin. By not washing, all stains will remain crystal violet purple, and none will appear safranin red/pink.
