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isotonic: to make sure the cells dont burst due to net flow of water into them that a hypertonic buffer can cause, or shrivel up, which a hypotonic buffer can cause.

buffered: to make sure the chloroplasts are kept at their physiological pH, which ensures that the key enzymes, proteins and other components of the cholorplasts retain their functionality.

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Q: Why is an isotonic buffered extraction medium used initially to isolate the chloroplasts?
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