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because it want to show that Sudan iv is soluble in water n not soluble when there is lipid presence . From there you can make lipid as your responding variable

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Q: Why distilled water is tested with Sudan IV in experiment of test for lipid?
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What chemical property allows lipids and Sudan IV dye to mix?

The sudan dye is a lipid itself, therefore you have a lipid layer interacting with another lipid.


Which test uncoated paper test or Sudan test is the most sensitive to small quantities of lipids?

for me, i think the uncoated paper test is more sensitive than Sudan iv test. This is because Sudan iv test can only soluble in certain solvent thus make it harder to define either the solution that is tested is really have lipid in it although there is not. The uncoated paper test can show if there is lipid the the solution if greasy transparent spot comes out from the uncoated paper.


Simple experiment determine if white substance is a lipid or carbohydrate?

A Simple experiment to determine if a white substance is a lipid or a carbohydrate is to rub it on a brown paper bag. If it leaves a greasy spot its a lipid.


What is the limitation for the lipid test?

Sudan IV is only soluble in certain solvents.


How Sudan IV detect the presence of lipid?

The chemical Sudan IV is not soluble in water; it is, however, soluble in lipids. Therefore to test for the presence of lipids in a solution you will use a Sudan IV Test. In this test dark red Sudan IV is added to a solution along with ethanol to dissolve any possible lipids. If lipids are present the Sudan IV will stain them reddish-orange, giving a positive test.


How do you use the Sudan IV test?

Sudan IV is a diazo dye for staining lipids, lipoproteins, and triglycerides. It is used in Sudan staining, wherein it attaches to the fats chemically.


What color does Sudan IV stain lipid?

it produces a brick/dark red stain.


Is distilled a lipid?

No. The term "distilled" is an adjective used to refer to a substance that has been purified by boiling it to separate it from less volatile impurities. For example chemistry labs often use distilled water.


Why does lipids turn redish brown when added to Sudan iv dye?

Sudan dye is lipid -soluble dye, thus, it will turn any lipids to red. It mixes with it.


What is a positive Sudan IV test result?

If no lipids are present, the red dye will sink to the bottom of the test tube. Sudan (iv) is not soluble in water but soluble in lipid.


What does a pink color in the lipid digestion experiment indicate?

digestion has occured :)


What is an experiment that can determine whether a solid white substance is a lipid or a carbohydrate?

Rub the substance on a paper bag, if it leaves a grease stain, it is a Lipid.