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You will do this regardless since Indole is part of the SIM media....this media will turn black if H2S is present and you read it for motility just like standard motility media. It must incubate for 24-28 hrs before doing the indole portion. Hope this helps.

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Why anthrone reagent needs to be kept cold before using?

Anthrone reagent is prepared in concentrated sulfuric acid. Adding reagents to acid causes immediate dissociation of the reagent and releases heat. If your solution weren't cold, it could generate enough heat to blow out of the flask or bottle.


How will you be able to use the heights of precipitate to determine the limiting reagent?

To determine the limiting reagent using the heights of precipitate, you can first measure the height of the precipitate formed in a reaction. By comparing the heights of precipitate from different trials with varying amounts of reactants, you can identify which reactant runs out first. The limiting reagent will correspond to the trial with the least height of precipitate, indicating that it was consumed completely before the other reactants. This method allows for a visual and quantitative assessment of the limiting reagent based on precipitate formation.


Why are solutions heated before evaporation?

well i THINK heating the solution will speed up the reaction in the solution before adding the precipitate since the final observation required is the precipitation. you dont add the reagent before heating since it may give a false observation after heating, it might not precipitate normally but since you've otherwise it might. BY : RAY LEE SEACREST


How is ninhydrine reagent prepared?

Ninhydrin reagent is prepared by dissolving ninhydrin powder in a solvent such as ethanol or acetone. The solution is typically heated gently to aid dissolution. It is important to prepare fresh ninhydrin reagent before use to ensure its effectiveness in detecting amino acids.


How is anthrone reagent stored?

Anthrone reagent should be stored in a tightly sealed container away from direct sunlight and heat sources, at room temperature. It should also be kept away from incompatible substances and moisture, and monitored for any signs of degradation or contamination before use.

Related Questions

Why anthrone reagent needs to be kept cold before using?

Anthrone reagent is prepared in concentrated sulfuric acid. Adding reagents to acid causes immediate dissociation of the reagent and releases heat. If your solution weren't cold, it could generate enough heat to blow out of the flask or bottle.


How will you be able to use the heights of precipitate to determine the limiting reagent?

To determine the limiting reagent using the heights of precipitate, you can first measure the height of the precipitate formed in a reaction. By comparing the heights of precipitate from different trials with varying amounts of reactants, you can identify which reactant runs out first. The limiting reagent will correspond to the trial with the least height of precipitate, indicating that it was consumed completely before the other reactants. This method allows for a visual and quantitative assessment of the limiting reagent based on precipitate formation.


What is the limiting reagent when 150.0g of nitrogen react with 32.1 g of hydrogen?

To determine the limiting reagent, calculate the moles of each reactant: 150.0g nitrogen is 5.36 moles and 32.1g hydrogen is 31.8 moles. Using the balanced chemical equation, you can see that nitrogen is the limiting reagent because it will be completely consumed before all the hydrogen is reacted.


How do you prepare Schultz Reagent?

To prepare Schultz reagent, mix one part of concentrated sulfuric acid with nine parts distilled water. Allow the mixture to cool before use. This reagent is commonly used for the detection of proteins in biological samples.


How can you prepare borsches reagent in the lab?

To prepare Borsches reagent in the lab, mix equal volumes of concentrated sulfuric acid and glacial acetic acid in a fume hood. Then, slowly add 9 parts of water to the mixture while stirring constantly. Allow the reagent to cool before use.


Why can't you prepare and store tollens' reagent in advance for tests?

Tollens' reagent is made by mixing two solutions - silver nitrate and ammonia - right before use because the reaction is sensitive to light, air, and temperature, which can degrade the reagent quickly. Preparing it in advance may lead to a loss of effectiveness, affecting the results of tests. Therefore, it is best to prepare Tollens' reagent fresh when needed for accurate testing.


HOW LONG ago is 480 BC from 2010?

480 BC was 2490 years before 2010. You can determine this by adding the current year to the BC year, 2010+480= 2490.


Do you have to estimate before adding?

No.


Why are solutions heated before evaporation?

well i THINK heating the solution will speed up the reaction in the solution before adding the precipitate since the final observation required is the precipitation. you dont add the reagent before heating since it may give a false observation after heating, it might not precipitate normally but since you've otherwise it might. BY : RAY LEE SEACREST


What did you learn from adding and subtracting fractions?

I learned to always change the denominators before adding or subtracting the numerators. You must always have a common denominator before adding or subtracting.


Do you boil water before adding pasta to cook it?

Yes, it is recommended to boil water before adding pasta to cook it.


Write the word in which the consonant was doubled before adding -ing?

Write the word in which the consonant was doubled before adding -ing.