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Rf value.

polarity of solvent

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10y ago
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14y ago

Chromatography is a technique that separates molecules from each other on the basis of their solubility in particular solvents. As a nonpolar solvent moves up the chromatography paper, the pigment moves along iwth it. The more non-polar a pigment, the more soluble it is in a nonpolar solvent, and the faster and father it proceeds up the chromatography.

Pg 94, laboratory 8.1, Inquiry into Life, Sylvia S. Mader, laboratory manual, 12th edition

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12y ago

Some factors that affect how far a pigment travels up the chromatography strip are the polarity of the molecules in the solvent (dictating how willing they are to travel with the solvent up the paper) solubility of the solvent (the less soluble molecules will drop out of the solvent more quickly), and the size of the molecule (the larger molecules will be less likely to travel far up the strip being carried by the solvent).

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13y ago

one factor is the solubility of a substance in that solvent the other i donot know

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12y ago

the solubility and the molecule size of the pigments

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11y ago

Polarity and molecular mass

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7y ago

Edward Brooke

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Q: What two factors determine the rate at which the pigments travel up the chromatography paper?
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Related questions

Why does carotene travel farthest in chromatography of leaf pigments?

it has more affinity (attraction) to the solvent than to the paper


What property of the ink was used to separate the colours?

The process is called chromatography. The property is the attraction of pigments to a liquid or solution (such as water) and its ability to dissolve in the solution. In paper chromatography, a pigment that dissolves easily in water will travel farther up the paper as opposed to an oil, that does not dissolve in water.


How is chromatography used to study plant pigment?

chromatography is basically a technique used for the separation of different components.... plant pigment consist of different components..... the sample is taken nd spotted over a chromatography paper..... nd den it is kept in suitable solvent to get separated...... different components travel different distance on chromatogram.... dis is how v cn use chromatography to study plant pigments...


What are the relations with pigment and chromatography?

the more soluble the plant pigment is with the chromatography solvent, the farther it will travel up the chromatography paper


What are the factors which determine the distance that a projectile will travel?

gravity - velocity - windage - obstructions


Why do pigments travel different distances up filter paper?

It depend on the density of the pigments.


Why do different substances travel at different speeds in a chromatography?

yes they do


Why do different colors travel at different speeds?

The paper is made of cellulose, a polar substance, and the compounds within the mixture travel farther if they are non-polar. More polar substances bond with the cellulose paper more quickly, and therefore do not travel as far. found on <http://en.wikipedia.org/wiki/Chromatography>


Why does blue travel further than red in chromatography?

Because blue looks better than red


What effect would doubling the length of the chromatography paper have on the Rf values?

Not much, the separation in Paper Chromatography could be improved but also the diffusion (vagueness of the spots) and the run time.In rising P-Chrom. the capillary forces may become too weak to go up further, and evaporation also can have negative effects. This can be omitted by hanging P-Chrom.


Why does red ink travel further than yellow ink in chromatography?

because yellow is a bigger particle, and in chromatography, the bigger molecules/particles fall out first, while the smaller particles continue moving.


What is paper chromotogrophy?

Paper chromatography is an analytical method technique for separating and identifying mixtures that are or can be colored, especially pigments. This can also be used in secondary or primary colours in ink experiments. This method has been largely replaced by thin layer chromatography, but is still a powerful teaching tool. Double-way paper chromatography, also called two-dimensional chromatography, involves using two solvents and rotating the paper 90° in between. This is useful for separating complex mixtures of compounds having similar polarity, for example, amino acids. If a filter paper is used, it should be of a high quality paper. The mobile phase is developing solutions that can travel up to the stationary phase carrying the sample alongside with it.