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What is the reason for not getting DNA bands after electrophoresis?
Wiki User
∙ 15y ago
A few reasons you may not see bands on the gel after electrophoresis:
- DNA concentration too low. More sample has to be loaded
- DNA sample is contaminated with RNA
- DNA bands are too small and have run out of the gel
- The potential (voltage) applied across the gel is not strong enough
- The buffer system in which the gel is suspended is not doing its job correctly. The buffer might have to be made fresh.
- The electrophoresis apparatus is not in the ocrrect orientation (electrodes not connected to the right poles)
Additionally, there could also be other reasons like: improper DNA extraction procedure. If you are running a gel after PCR and still do not see bands, look into whether the DNA is being amplified correctly. See if you are using the correct primers.
There are several factors that influence the electrophoresis technique. A close examination of the results obtained will help you make decisions about your future experimental approach.
Wiki User
∙ 15y ago
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When is DNA cut during electrophoresis?
Electrophoresis technique is not designed to cut DNA molecule. When DNA is analyzed by electrophoresis to determine its molecular mass, the molecular biology engineer usualy digests the DNA molecule, before the electrophoresis, with specific enzymes called "restriction enzymes" in order to obtain fragments of diverse molecular weights that can be seen as bands in electrophoresis gels.
What is the pattern of dark bands on photographic film that is made when DNA fragments are separated by gel electrophoresis and tagged?
DNA fingerprint
How do you avoid smearing of DNA bands in gel electrophoresis?
Make sure the voltage does not run over 200.
If all the bands on an electrophoresis gel are the same color the single stranded DNA sample consisted of one kind of?
false
DNA samples taken from Dolly and the sheep that donated the body cell would show what patterns of bands on an electrophoresis gel?
DNA samples taken from Dolly and the sheep that donated the body cell showed the same patterns of bands on an electrophoresis gel. Dolly became the first cloned mammal which then led to the cloning of pigs, dogs, and mice.
What is the role of EtBr in electrophoresis?
Ethidium bromide interchalates with DNA. It doesn't affect electrophoresis, but it help visualise the DNA bands after electrophoresis. The EtBr that is bound to the DNA will fluoresce under ultraviolet light.
Are restriction enzymes used in gel electrophoresis?
For DNA gel electrophoresis, yes. Once the DNA is cut up into different-sized fragments, they can be electrophoresed to separate bands.
When is DNA cut during electrophoresis?
Electrophoresis technique is not designed to cut DNA molecule. When DNA is analyzed by electrophoresis to determine its molecular mass, the molecular biology engineer usualy digests the DNA molecule, before the electrophoresis, with specific enzymes called "restriction enzymes" in order to obtain fragments of diverse molecular weights that can be seen as bands in electrophoresis gels.
What is the pattern of dark bands on photographic film that is made when DNA fragments are separated by gel electrophoresis and tagged?
DNA fingerprint
How do you avoid smearing of DNA bands in gel electrophoresis?
Make sure the voltage does not run over 200.
What is role of agarose of electrophoresis?
agarose helps in the separation of DNA bands by controlling the pore size of agarose gel
If all the bands on an electrophoresis gel are the same color the single stranded DNA sample consisted of one kind of?
false
DNA samples taken from Dolly and the sheep that donated the body cell would show what patterns of bands on an electrophoresis gel?
DNA samples taken from Dolly and the sheep that donated the body cell showed the same patterns of bands on an electrophoresis gel. Dolly became the first cloned mammal which then led to the cloning of pigs, dogs, and mice.
What kind of pattern of bands on an electrophoresis gel would show from the DNA samples taken the Dolly?
They would be the same since Dolly is clone.
Why does the the florescense appear in the chaneels only n not in the form of moving bands While performing Gel electrophoresis For DNA detection?
Only the bands should show fluorescence, you must be doing the technique wrong..
What is used to sort DNA into different lenghts?
Gel Electrophoresis
What are the common stains that is used after DNA electrophoresis?
Ethidium bromide and coomassie blue are some stains that can be used in DNA electrophoresis.
