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What is the advantages and disadvantages of pBR322 as a cloning vector?
Wiki User
∙ 11y ago
pBR322 advantages is it widely used for the analysis of prokaryotic transcription and translation as well as topological changes in DNA conformation.
then the disadvantage is it has only few cloning sites and the selection procedure is therefore time consuming.
Wiki User
∙ 11y ago
Anonymous
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What is the cloning capacity of pBR322 vector?
The cloning capacity of pBR322 vector is 1-5kb.
Explain the functioning of pBR322 vector with diagram?
FUNCTIONG OF pBR322
WHAT IS pBR322?
pBR322 is one of the most used cloning vectors in molecular biology. Cloning vectors, best-known as plasmids, are autonomously replicating DNA units into which DNA fragments can be inserted for gene cloning. Genes taken up by these plasmids are multiplied (or cloned) as the vector replicates, to yields numbers suitable for molecular analysis. The most versatile and well-known plasmid is certainly pBR322 (in fact was one of the first ever used in gene cloning techniques) and has genetically tailored cutting sites into which DNA can be inserted without affecting plasmid self-replication. pBR322 general characteristics are: a) Size: 4.3 kb; b) Replicon: ColE1, relaxed; c) Selective markers (resistance): Amp and Tet; d) Single sites (enzymatic restriction single sites): Ava I, Pst I, BamHI, PvuII, ClaI, SalI, EcoRI, and HindIII.
Is a plasmid a cloning vector?
i have no idea wat plasmid,cloning ,and vector means so haha 2 u
What is the advantages and disadvantages of vector scan disply?
Advantages of vector scan display include high resolution, smooth lines, and efficient use of memory. Disadvantages can include limited color capability, complexity in generating images, and susceptibility to distortion with complex shapes.
Is a cloning vector a plasmid?
plasmid is the type of the cloning vector. other cloning vectors includes cosmids, bacteriophage, phagemids, artifiical chromosomes. clonong vectors are the carriers of certain traits to be inserted in non coding regions of the DNA.
Why is cloning an example of cloning?
Gene Cloning is used to clone a gene of interest in a vector called plasmid. The chimeric DNA or rDNA formed by cloning is stable and can be used to propagate and sequence the DNA. producing vector containing inulin gene is an example.
What is the insert capacity of a cosmid vector in DNA cloning?
The insert capacity of a cosmid vector is about 35-45 kb.
What a good cloning vector should have?
The good cloning vector should have small size, high copy number, own origin of replication, restriction sites for many restriction enzymes and selectable markers.
How can you know donored-vector is cloning vector or expression vector?
Expression vectors contain translation start and termination codons. Moreover, it contains promoter region
The host organism into which a cloning vector is placed is called?
Competent cells if i not mistaken
Does a cloning vector contain promoter region?
No, not really since it is just for cloning. But their should be enough promoter/sequence to provide antibiotic resistance.
