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Tailing peaks are a known problem in chromatography (both HPLC and GC). It means the analytes are experiencing an out-drawn (extended) retention time and is usually due to one of these factors:

  • Contaminated or active injector liner, seal or column
  • Dead volume due to poorly installed liner or column
  • Ragged column end
  • A bad match between the polarities of the stationary phase and the solvent
  • A cold region in the sample flow path (GC)
  • Debris in the liner or column
  • Injection takes too long
  • Split ratio is too low (GC)
  • Overloading the inlet
  • Some types of compounds such as alcoholic amines, primary and secondary amines and carboxylic acids tend to tail
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Q: What is tailing in chromatography?
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