Mannitol Salts agar is a selective and differential media used to identify Staphylococcus sp. The media is selective due to the high salt concentration which prevents all but halophiles from growing. The media is differential due to the presence of mannitol and the indicator Phenol Red. Staphylococcus aureus can ferment mannitol and produce lactic acid as a result. The media contains an indicator called Phenol Red which turns yellow in low pH environments. This results in a yellow halo around the S.aureus colonies. Staphylococcus epidermidis cannot ferment mannitol and so the colour of the media around these colonies does not change. Certain vibro species and other halophiles may grow on these plates some of these appear pink and some may be able to ferment mannitol leading to false positives. S.epidermidis is a normal commensal organism and grows on the skin. S.aureus is a potential pathogen, antibiotic strains exist and can be hospital accquired infections. Search for "MRSA" for more information.
Mannitol salt agar is selective for gram positive bacteria, and differential for mannitol fermenters. Phenol red is the indicator containing the enzyme mannitol.
In my result i isolate bacillus sp on mannitol salt agar but i expect that this media has deoration or expaired
If the salt concentration is high as in mannitol salt agar 7.5% NaCl, this will inhibit the growth of gram positive bacteria.
Mannitol Salt Agar Complex is a synthetic medium because it is prepared from pure chemical substances and the exact composition of the medium is known.
Mannitol salt agar supports growth of organisms that can grow in a high salt concentration, particularly Staphylococcus species and halophiles. The phenol red pH indicator in the agar will also let you know whether or not the bacterium you streaked ferments mannitol by changing to a yellow color if fermentation has occured.
Mannitol salt agar is selective for gram positive bacteria, and differential for mannitol fermenters. Phenol red is the indicator containing the enzyme mannitol.
In my result i isolate bacillus sp on mannitol salt agar but i expect that this media has deoration or expaired
If the salt concentration is high as in mannitol salt agar 7.5% NaCl, this will inhibit the growth of gram positive bacteria.
Mannitol is not a substrate for glycolisis
Gram-positive bacteria grows in Mannitol agar because it contains a high level of salt. This type of agar allows only certain types of bacteria to grow, making it selective.Ê
the salt content
Mannitol Salt Agar Complex is a synthetic medium because it is prepared from pure chemical substances and the exact composition of the medium is known.
Both
it is selective because only a salt tolerant can grow. 7.5 nacl it has diffrential properties also. if mannitol formentors turns yellow staphlococus aureus; but if it has no change then its a nonpathogenic staphlococci (s. edermis)
Mannitol salt agar (MSA) contains high levels of salt because it inhibits the growth of most bacteria. This makes it an excellent medium to test for Staphylococci and Micrococcaceae as they are tolerant of high levels of NaCl.
Mannitol salt agar is used for the isolation of staphylococci which is found normally on skin (S. aureus). The selectivity is obtained by the high salt concentration that inhibits growth of many groups of bacteria.
No, K. pneumoniae will not grow on Mannitol Salt Agar- gram negative organisms cannot grow on MSA, and K. pneumoniae is gram negative.