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What is enzyme coupling?

Updated: 10/31/2022
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11y ago

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Some enzyme-catalyzed reactions do not involve changes in substrates, products, or cofactors that have easily observable properties (e.g. changes in light absorbance, etc) for the measure of reaction kinetics. In such cases, additional enzymes may be included in the reaction mixture that catalyze a reaction using the product of the first enzymatic reaction as a substrate, metabolizing it to a compound that has more easily measurable properties. If this second reaction is much faster than the first, the kinetics of the overall path approximate the kinetics of the slower reaction alone. This technique can also be used to move spectral peaks of a substrate farther away from those of interfering species, such as peaks normally observed around 280 nm for proteins (due mostly to absorbances oftryptophan, tyrosine and cysteine residues)

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