tube coagulase is done in test tube while slide coagulase is done on slide.thnk you all!
cheeres!
YES
Rabbit plasma, sometimes with EDTA added
staph. aureus test positive for catalase test and coagulase test consecutively
Gram + coccus. Positive methanol test. Yellow on agar.
No, Catalase +ve, Coagulase +ve, Gram + cocci. Citrate test is usually used to differentiate members of the Family Enterobacteriaceae. Check Bergeys systematic bacteriology. 1980
Coagulase is a protein enzyme. It is important to use fresh cultures in a coagulase test because coagulase expression is highest during the log phase of growth.
YES
widal slide is easier and it save time but widal tube test is not easy and it takes time.
Staphaurex is a rapid slide agglutination procedure for differentiating Staphylococci that possess coagulase and/or protein A. Particularly Staphylococcus aureus, from Staphylococci that possess neither of these factors.
Rabbit plasma, sometimes with EDTA added
staph. aureus test positive for catalase test and coagulase test consecutively
what is the difference between proffiency and diagnostic test
what is the difference between proffiency and diagnostic test
The difference between a test and a demonstration is that a test is to be taken and answered and a demonstration is to be demonstrated and be told to the class
Free coagulase is an extracellular toxin that reacts with a thrombin-like, thermostable substance known as coagulase-reacting factor (CRF) or serum factor. The reaction of coagulase and CRF will lead to the formation of coagulase-CRF complex where this complex indirectly changes frbrinogen to fibrin (clotting factor) and hence will form a fibrin clot. Furthermore, it is also use as the primary indicator of virulence among staphylococci. Its clinical significance is that when doing the coagulase tube test, the free coagulase will react with the plasma in the tube and hence convert the fribrinogen into fibrin which lead to fibrin clot formation in the tube. This is shown after one day of incubation of the tube, when the tube being invert, the plasma will not go down showing that it is clotted.
in the coagulase test, normally a virulent staphylococcus is put into plasma. plasma contains the plasma protein fibrinogen. when fibrinogen is acted upon by a fibrinogenase, it is then converted to fibrin. fibrin has te ability to form clots. so in the case of staphylococcus aureus which has the enzyme coagulase also called fibrinogenase, the enzyme acts on the fibrinogen in plasma which is converted to fibrin hence the clots that are seen suspending in the solution after 4 hours.
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