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What is cell seeding density?

Updated: 8/11/2023
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14y ago

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Cell seeding density refers the number of cells (usually per square centimeter) that are placed into a flask during cell passage. If growth is wanted a typical cell seeding density would be around 5,000 cells/square cm, however, if a high confluence is needed it may be more appropriate to seed cells around 50-60,000 cells/square cm.

How to determine cell seeding density-

look at the cells in a hemocytometer. This is a small plate that has very small squares etched into its surface, which allows for counting of individual cells underneath a microscope to determine the density of the cells in the cell suspension, and this the total number of cells can be approximated.

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