What is Fluorescence microscope?

Answer 1

A fluorescence microscope uses a much higher intensity light to illuminate the sample. This light excites fluorescence species in the sample, which then emit light of a longer wavelength. A fluorescent microscope also produces a magnified image of the sample, but the image is based on the second light source -- the light emanating from the fluorescent species -- rather than from the light originally used to illuminate, and excite, the sample. - I found a great link that compares this to conventional microscopes. Click the link below!

Answer 2

normally fluorescence microscope is one of the three type of light microscope, it is used to observe luminous substances which tend to absorb invisible UV light and radiate a visible spectrum of different colours such as green red and yellow. there for different parts of the cell absorbs UV light and radiate visible radiation of different colour and hence can be viewed.

Answer 3

First antibodies are created that bind a single protein. Then the antibodies are connected to a chemical compound that lights up with a specific color upon being excited by light of a certain wavelength. Each antibody has a different color molecule so if a cell is treated with one that binds say the nucleus and another that binds the rough ER you can see them as different colors under a microscope. The target that is bound by the antibody is usually a organelle or protein that is not visible under normal microscope conditions. So once bound the target to be visualized the antibody/chemical is "excited" by wavelengths of light that cause the chemical to target to light up with a specific color. A picture is taken and you can now see a part of the cell that is normally invisible.