What are the two basic principles involved in using a microscope?

Answer 1

principle of microscopy includes 2 important aspects:

1. resolution and resolving power- the resolving power of a microscope is defined as the minimum distance by which 2 points must be separated in order for them to be perceived as 2 separate points rather than a fused image.

2. magnification- Linear (sometimes called lateral or transverse) magnification refers to the ratio of image length to object length measured in planes that are perpendicular to the optical axis. A negative value of linear magnification denotes an inverted image. Longitudinal

magnification denotes the factor by which an image increases in size, as measured along the optical axis.

Answer 2

One principle of operating a microscope is that there is more than one lens that can be used. Another principle of operating a microscope is that items to be looked at should be in a saline solution to help see them more clearly.

Answer 3

In a simple microscope there is only 1 lens and 7 cm tall and there is a compound microscope where the image from objective lens is magnified again by the ocular lens used mainly for slides, in schools, labs, there are variations like brightfield illumination and darkfield illumination compound microscopes. Fluorescence microscope uses uv light and cells stained with fluorescent dyes. Confocal microscope uses fluorescence colors and laser light producing a 3d image. Scanning Acoustic microscope uses a computer and measures sound waves reflected back from object and it is used to study cells attached to a surface. Electron microscopes uses electrons instead of light and gives a greater resolution there are 3 kinds, TEM uses dead, ultrathin sliced specimens stained with heavy metal salt, SEM uses an electron gun that scans whole specimen and gives a 3d image and a Scanning-Probe microscope uses a metal probe to scan specimen and creates a 3d computer generated picture of specimen.

Answer 4

when there is a base of black so that organele can b appereqared

Answer 5

The two basic principles that are involved in using a microscope are magnification and observation. There are different types of microscopes which mainly include optical and electron.

Answer 6

1. At least two adjustable lenses

2. A light source to see the image

Answer 7

The two basic principles for using a microscope would make sure its on and slide is in place. Focusing in on the area of the slide using the large knob then the smaller knob.

Answer 8

The two basic principles that are involved using the microscope are magnification and observation. They are both done by examining an object through the lens of the microscope.

Answer 9

Basic technique in microscopy makes use of the differences in refractive index of cell structures.