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The use of an antibiotic resistance gene on a plasmid used in genetic engineering makes?
Wiki User
∙ 13y ago
direct selection possible
Wiki User
∙ 13y ago
Wiki User
∙ 13y agoDirect selection possible
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In genetic engineering what takes up plasmid?
In genetic engineering, the bacterial cell takes up the plasmid
Are plasmid vectors helpful?
Plasmid vectors are an invaluable genetic engineering tool for inserting recombinant DNA sequences into different organisms or cells in culture.Plasmids are essentially circular DNA constructs composed of some essential elements like:An origin of replicationA multiple cloning site which consists of restriction sites where the recombinant DNA can be insertedMarker genes (like antibiotic resistance)reporter genes to confirm a successful transformation
How does the ti plasmid make genetic engineering plants a possibility?
It is capable of introducing exogenous genes into plant genomes. T-DNA genes are removed from the Ti plasmid and are replaced with the gene of interest.
A eukaryote used in genetic engineering is?
Virus and plasmid. Both can insert the target gene into the host's genome.
How could you use genetic engineering techniques to make transformed bacteria that produce the enzyme?
Extract DNA from the cells of people who can make the digestion enzyme. Cut the DNA with restriction enzymes to cut out the gene that codes for the enzyme. Use gel electrophoresis to locate the gene. Then, use polymerase chain reaction to make copies of the gene. Choose a plasmid that has an antibiotic-resistance genetic marker, and cut the plasmid with the smae restriction enzyme use to cut out the hyman gene. Insert the copies of the human gene into the plasmids. Allow bacterial cells to take in the plasmids. Select for transformed bacteria by growing them in a culture containing the antibiotic. These bacteria will make the digestion enzyme.
In genetic engineering what takes up plasmid?
In genetic engineering, the bacterial cell takes up the plasmid
What type of plasmid is the source of the problem with antibiotic resistance?
R plasmid.
What structures contains genes for enzymes and antibiotic resistance?
plasmid
Is it true cells containing a plasmid with an antibiotic resistance gene will die in the presence of the antibiotic?
false
What is an extra loop of DNA that contains antibiotic resistance what gene?
Plasmid
What type of gene is used to distinguish bacteria that carry a plasmid containing foreign DNA from those that don' t?
The plasmid that contains foreign DNA is engineered to also carry an antibiotic resistance gene. This antibiotic resistance gene codes for a protein that is able to inactivate an antibiotic thus keeping the cell alive. In the absence of the antibiotic resistance gene, the cells would not survive when exposed to an antibiotic. After transfection (the process of inserting the plasmid carrying the foreign gene into cells), the cells are gown in media containing an antibiotic. Cells that contain the plasmid (and therefore contain the antibiotic resistance gene) are able to survive in this medium. Cells that do not contain the plasmid (and therefore lack the antibiotic resistance gene) do not survive in this medium. The process described above is called selection
True or false cells containing a plasmid with an antibiotic resistance gene will die in the presence of the antibiotic?
false
How does plasmid allow for antibiotic resistance?
A plasmid (conjugative plasmid) that has a resistance gene on it, can transfer itself to another bacterial cell (called conjugation) or assist in the transfer of a non-conjugative plasmid that has a resistance gene to another cell (called mobilization). Whichever way it happens, once the plasmid is transfered to the new cell, this cell too may show signs of resistance to the particular antibiotic. This is one of the ways of the speard of resistance amongst bacteria
Why are plasmids pieces of DNA?
i think each plasmid piece codes for a specific function. for example antibiotic resistance shown by antibiotic sensitive cell after a piece of plasmid that is antibiotic resistant gene recombine with cell DNA.
What is one way to determine whether a bacterial culture has received a recombinant plasmid?
The plasmid have a "reporter gene" inside it, generally resistance to specific antibiotic. the plasmid is transformed into bacteria that don't have resistance to that specific antibiotic drug, and than the cultured on a petri-dish that contain the antibiotic drug. Only bacteria that had receive the plasmid will have resistance and grow, all the other will die.
How will you identify cells carrying the recombinant plasmid?
The transformants are selected for on agar containing an appropriate antibiotic. For example if your recombinant plasmid contains a kanamycin cassette, then only the cells containing the plasmid will grow on an agar plate containing kanamycin. PCR can then be performed on the colonies to ensure they contain your gene of interest on the plasmid.
Are plasmid vectors helpful?
Plasmid vectors are an invaluable genetic engineering tool for inserting recombinant DNA sequences into different organisms or cells in culture.Plasmids are essentially circular DNA constructs composed of some essential elements like:An origin of replicationA multiple cloning site which consists of restriction sites where the recombinant DNA can be insertedMarker genes (like antibiotic resistance)reporter genes to confirm a successful transformation
