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Perhaps, but several issues arise. If the lion was the female (being the lioness), she would probably end up with a torn vagina because a buck deer's penis is about 5 times as long as the lions. Plus the buck likely ejaculates much more and a bit more forcefully than a lion, and has a more powerful orgasm, so she could get injured.

If it was vice versa, the doe would probably get nerve damage in her hind legs or injure her hind legs or pelvic region from having to support an animal that is three times her mate's weight. A lion's penis also has tiny barbs on it which would likely cause just a bit of damage to the doe's vagina. See related links below for images of lion and deer penises.

Another issue is that lions and lionesses mate with the lioness crouching down with her belly touching the ground, with the lion mounting her in such a way that he doesn't need to put much weight on her back, if at all. Deer, on the other hand, mate standing up, with the buck putting a third of his weight on the rear hindquarters of the doe as he thrusts himself in and ejaculates. These differences in methods of breeding could make for some very awkward and confusing attempts of either party, no matter if it's a buck to a lioness or a lion to a doe.

The other possibility is that there would be no mating involved and the lion would just rather kill and eat the deer than mate with it.

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Q: Is it possible for a lion and a deer to mate?
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