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No. safranin is the classic stain used in gram staining. Concentrated Carbol Fushin is mainly used for the ZN staining procedure to stain organisms such as Vibrio cholerae and Cryptosporidium. Diluted Carbol Fushin can however be used as a replacement counterstain for Safranin in the gram stain.
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What is the purpose of heating slide with carbol fuchsin?
to break down the cell wall
Difference between hot method and cold method of acid fast staining?
The hot method uses heat to force the primary stain (carbol fuchsin) into the cell wall, while the cold method uses a detergent to cause the stain to penetrate the wall.
Why was safranin use for the cheek cell?
We used safranin on how to essilly see the specimen.
Is safranin an acidic dye?
Sure, any basic stain can be used for simple, direct staining.
What chemical is responsible for the acid fast property of mycobacteria?
the cell wall contains mycolic acid. a dye (carbol fuchsin) is applied to the culture, then washed with acid-alcohol. those cells with mycolic acid in their cell wall will retain the dye even after the alcohol rinse. but those cells without mycolic acid will release the dye.
What is the purpose of heating slide with carbol fuchsin?
to break down the cell wall
Name three basic stains?
methylene blue crystal violet carbol fuchsin
What type of dye is used to stain the specimen when the acid-fast stain and the gram stain are used?
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
What kind of stain do you stain Mycobacterium with?
For Mycobacterium you will use the Acid-fast staining technique. There are two different methods of stainging: 1) Ziehl-Neelsen Method and 2) Kinyoun Method.1) The Ziel-Neelsen method uses a primary stain of Carbol Fuchsin dye that must be steam treated, rinsed with acid alcohol wash, and a secondary stain of Methylene Blue.2) The Kinyoun Method uses a primary stain of Kinyoun Carbol Fuchsin dye that is not steam treated. An acid alcohol wash is applied and a secondary dye of Brilliant Green. This technique is called "cold staining".The mycolic acid within the Mycobacterium cell membrane has a high affinity for the Carbol Fuchsin dyes.
What is gram stain reaction for Borrelia Burgdoferi?
when stained with Gram stain Borrelia take up the counter stain which is carbol fuchsin or safranin and they appear as Gram negative spiral rods in gram film. In order to stain them the time required for staining them is little bit more as compared to normal gram staining. The initial steps are the same but once you apply the counter stain leave it for a while may be 5-10 mins depending upon the strength of counter stain. After washing the slide and drying once can see them on oil immersion lense.
When was Leo Carbol born?
Leo Carbol was born in 1910.
When did Leo Carbol die?
Leo Carbol died in 1991.
Difference between hot method and cold method of acid fast staining?
The hot method uses heat to force the primary stain (carbol fuchsin) into the cell wall, while the cold method uses a detergent to cause the stain to penetrate the wall.
What color is safranin?
Safranin is a light pink/red color.
What is the decolorizing agent in the acid fast stain?
The decolorizing agent in the acid fast stain is acid alcohol. The decolorizing agent in the gram stain is ethanol.
Why was safranin used for cheek cell?
We used safranin on how to essilly see the specimen.
Why was safranin use for the cheek cell?
We used safranin on how to essilly see the specimen.
