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How does BAPNA work?

Updated: 10/31/2022
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12y ago

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Trypsin is a pancreatic enzyme that hydrolyzes proteins. BAPNA is a synthetic dye covalently bonded to an amino acid. The solution is colorless but turns yellow when it is hydrolyzed. Since the covalent bond in BAPNA is the same as the covalent bonds found between amino acids within a protein, a positive hydrolysis reaction would indicate that Trypsin has separated the bond between the dye and the amino acid in BAPNA.

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