Polyclonal antibodies
Monoclonal antibodies
Inexpensive to produce
Expensive to produce
Technology required is low
High technology required
Skills required are low
Training is required for the technology use
Time scale is short
Time scale is long for hybridomas
Produces large amounts of non specific antibodies
Can produce large amounts of specific antibodies but may be too specific
Recognizes multiple epitopes on any one antigen
Recognizes only one epitope on an antigen
Can be batch to batch variability
Once a hybridoma is made it is a constant and renewable source and all batches will be identical
In coomassie staining, a dye binds to all proteins, visualizing all of them. In this way, it's nonspecific. The dye diffuses into the the original gel. In a western, an antibody binds specifically to one or more proteins, making it specific for proteins to which the antibody can bind. In order for the antibody to access the proteins from the original gel, they are moved (blotted) to the surface of a membrane (usually nitrocellulose).
They both promote a specific chemical reaction, also a ribozyme can act as an enzynme and have the ability to replicate it's self. Answered by: Alyssa White
What is the difference between herds and packs?
Difference between collenchyma and chlorenchyma
what is the difference between 2x and 2n
polyclonal antobody is the antibody produced for many or non specific antigens but antiserum is the antibody for a specific antigen
Chickenpox and shingles result from the same virus, and generate the same antibodies. There is no difference between chickenpox antibody and shingles antibody, and there is only one test (varicella virus antibody) for both.
For information on this subject go to the related link (MedImmune Initiates First Clinical Trial of Monoclonal Antibody Targeting GM-CSFR in Patients with Rheumatoid Arthritis) Below
Antibodies lack a transmembrane domain.
Antigen binding site or epitope is a part of an antigen that is recognized by the antibody. Paratope is a part of an antibody that binds on epitope.
Opsonization involves an antibody binding to a pathogen itself while neutralizing invovles an antibody binding to a toxin, AKA something the pathogen produces.
the autoimmune reaction is the reaction between the antibody and antigene when both come from the same body but the isoimmune reaction is between the antigene and antibody produced for the same antigene ( the same according to the type but not the immunity )
difference between an antibody and an antigen
Antibodies are secreted (plasma cells), while immunoglobulins are membrane-bound (naive B cells).
Basically to explain this, an antigen is any type of pathogen that causes disease, while an antibody is something that combats against the antigen.
agglutination reaction
In coomassie staining, a dye binds to all proteins, visualizing all of them. In this way, it's nonspecific. The dye diffuses into the the original gel. In a western, an antibody binds specifically to one or more proteins, making it specific for proteins to which the antibody can bind. In order for the antibody to access the proteins from the original gel, they are moved (blotted) to the surface of a membrane (usually nitrocellulose).