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Nigrosin is a dye made of synthetic ingredients. it is a negative stain, which is what is required to be able to stain staphylococcus.
Wiki User
∙ 9y ago
Wiki User
∙ 9y agoYes, staphylococcus cells can be stained by nigrosin. This is because nigrosin is a black dye mixture usually found in varnishes and lacquers.
Wiki User
∙ 9y agoNigrosin is a mixture that is composed of synthetic black dyes. A staphylococcus cannot be stained using nigrosin because this bacterium carry a negative charge that repels nigrosin.
Wiki User
∙ 12y agoyes, it does. I've stained it in class before
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What would the slide look like under the microscope if methylene blue is used instead of nigrosin?
The stain would stain the cells rather than the background
Is counterstain a negative stain?
Yes. Eosin and acid fuchsin are also acidic dyes like nigrosin.
Methicillin-resistant Staphylococcus aureus gram stain?
Would be gram-positive because of the cell wall of the Staphylococci.
Is a stain on a microscope specimen used to turn it a pretty colour or not?
I like trains, more like
What describes the correct cellular morphology and gram reaction for staphylococcus aureus?
The cell morphology of Staphylococcus aureus is spherical in appearance. Spherical bacterium are known as coccus and appear as tetrads (4) or more in groups. They stain Gram +.
Are nigrosin and malachite green a functional analogous pair of stain?
No
Is it possible to substitute methylene blue for nigrosin in the negative stain?
no
What are the materials used in capsule stain?
For the capsule stain Congo red or a Nigrosin solution can be used. Next, Maneval's stain is used.
Why doesn't a negative stain colorize the cells in the smear?
A negative stain will stain the background with an acidic dye, such as Nigrosin. This procedure is used to demonstrate capsules. This technique brings the specimen off of the background for more adequate viewing purposes.
What would the slide look like under the microscope if methylene blue is used instead of nigrosin?
The stain would stain the cells rather than the background
Why cant methylene blue be used in place of nigrosin in negative staining?
Because negative staining requires the use of an acidic stain, which will not penetrate the cells because of the negative charge on the surface of the bacteria. As a result, the unstained cells can be easily identified against the colored background.
Describe the microscopic appearance of encapsulated streptococcus if stained with safranin?
Safranin (red) is used in gram staining and endospore staining as the secondary stain. Nigrosin is used in negative staining, staining only the background and not the bacteria. Therefore, the bacteria within the capsule would stain red from the safranin. (Like in endospore staining and negative gram staining, safranin would stain the bacteria red.) Nigrosin would stain the background of the organism just as it would in negative staining. Bacteria (within capsul): stained safranin red Capsule (outer layer of bacteria): clear Background of organism: stained dark with Nigrosin
What specimen from a TB patient would the acid fast stain be performed on?
The specimen is taken from TB lesion
Why does nigrosin not stain bacterial cells?
Because Nigrosin is an acidic dye--carries a negative charge--and repels against the negatively charged cell walls of most bacteria.
What is it called to add dye to a specimen under a microscope?
to stain.
Chemical sometimes used to make the specimen visible?
Stain
What is a consequence of not leaving a stain on the smear long enough?
The specimen will?ænot absorb adequate stain, resulting in little contrast. Thus making it difficult for identification of?ædifferent components of the specimen.
