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The first stain is crystal violet, next iodine is added which acts as a mordent. Then, alcohol is added which will wash away the purple color from any gram negative bacteria. The purple color will stay in the gram positive bacteria due to their thich peptidoglycan layer. Lastly, Safranin is added as a counter stain. Gram - is red Gram + is purple
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∙ 13y ago
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∙ 10y agoThis a differential stain. It can "show" what type of bacteria there are on the slide, that plus colony type can give clue as to the identity of the "bug". It is important because of the antibiotic which as to be given.
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∙ 8y agoThe Gram stain is considered a differential stain. A differential stain makes use of two or more dyes and distinguishes between two kinds of organisms or between two different parts of an organism. Staphylococcus aureus (boils, septic shock), Staphylococcus epidermidis (skin lesions), and Streptococcus mutans (tooth decay) are all Gram+.Treponema pallidum (syphilis) , Vibrio cholerae (cholera) and Yersinia pestis (plague) are all Gram-.
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∙ 12y agoThe primary stain in a gram stain is typically Crystal Violet
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∙ 11y agoOne could do a simple stain of methylene blue counterstained with eosin Y. Not sure what you are doing, though.
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∙ 13y agoYou have to use a heat-fixed specimen.
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∙ 13y agoEubacteria
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Why is safrinin used in gram staining?
as a couterstain
What is the name of the paper used to dry slides after gram staining?
Gram staining is used to identify whether a bacterium is gram positive or gram negative. Slides can be dried using filter paper or tissues. The technique is based on the reaction of stain that happens with the membrane of bacteria.
Is gram staining related to flagella?
No. It is a staining on the cell itself.
What type of gram is H1N1 bacteria gram-positive or gram-negative?
neither...it is a virus, and gram-staining, a microbiology tool, is not used to help identify or classify a virus
Is carbol fuchsin the same as safranin?
No. safranin is the classic stain used in gram staining. Concentrated Carbol Fushin is mainly used for the ZN staining procedure to stain organisms such as Vibrio cholerae and Cryptosporidium. Diluted Carbol Fushin can however be used as a replacement counterstain for Safranin in the gram stain.
Why gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Why is Gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Why gram staining is classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Why is safrinin used in gram staining?
as a couterstain
What is the name of the paper used to dry slides after gram staining?
Gram staining is used to identify whether a bacterium is gram positive or gram negative. Slides can be dried using filter paper or tissues. The technique is based on the reaction of stain that happens with the membrane of bacteria.
What bacterial staining technique is it when a basic dye is utilized to stain bacterial cells?
A basic dye used in gram staining is crystal violet.
Is gram staining related to flagella?
No. It is a staining on the cell itself.
Who developed Gram staining?
Gram staining was devised by Hans Christian Gram of Denmark in the 1800s. (1853-1938)
What type of gram is H1N1 bacteria gram-positive or gram-negative?
neither...it is a virus, and gram-staining, a microbiology tool, is not used to help identify or classify a virus
Why must young cultures be used when doing staining?
Young cultures must be used when doing a gram stain to get more accurate results. The cell was is the part of the bacterial cell that is most involved with gram staining because it holds the crystal violet.
What counter stain is used in gram staining?
safranin
Why is Iodine used in Gram staining?
Iodine is added as a mordant to enhance crystal violet staining by forming a crystal violet-iodine complex.
