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Mycobacteria do not "take" the stain - i.e - it will not color them. To see mycobacteria you need to use acid fast stain.

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โˆ™ 2013-01-05 19:59:19
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Q: Why you can not use Gram stain for diagnosis mycobacteria?
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Related questions

Is a gram stain a simple stain?

Gram stain is not a simple stain because simple stains do not use two or more stains. Gram stain is a differential stain differentiating between Gram positive (blue-black) and Gram negative (pink-red).


Why can't you use the gram stain on the pathogens?

Which pathogens can't you use the gram stain on? Some pathogens are gram negative such as Tuberculosis. Others are gram positive. Those you can stain with the gram stain. The differences have to do with the structure of the outer membrane. Not all pathogens are gram negatives. Staph and strep are gram positive. People die from Strep infections.


What is the advantage of the gram stain over simple stain such as methylene blue?

Gram staining highlights different bacteria types through the use of special dyes. It aids in the diagnosis of a specific organism and tells the difference between gram negative and gram positive bacteria. Simple staining is unable to highlight the exact organism.


Why is it necessary to use Safranin in gram stain?

Gago


What does gram negative on a serratia marcescens gram stain mean?

Bacteria are gram positive or gram negative. Serratia happens to be a gram negative bacteria. They appear pink on a gram stain. Gram positive bacteria stain to a purple color on a gram stain. We can classify and ID bacteria using their gram stain and shape. Some antibiotics only work on gram negative bacteria and some only work on gram positive bacteria. It helps a doctor know which antibiotic to use.


What is the use of Iodine in gram stains?

The iodine in the gram stain serves as a mordant or fixative.


What the purpose of gram staining?

First to stain the organism. Gram stain differentiates organisms in to gram positive and gram negative. This will help the microbiologist to choose which media to use to grow them, have a clue what might be the organism is, etc.


What happens if you use gram stain on a virus?

Most likely nothing. The gram stain will only stain the peptidoglycan cell wall of bacteria. Most viruses have a protein coat called a capsid. The capsid will not take up either the crystal violet or the safranin of a Gram stain.


What happens when you only use counter stain gram positive?

poop


What is the advantage of the gram stain over simple stains such as methylene blue?

Gram staining highlights different bacteria types through the use of special dyes. It aids in the diagnosis of a specific organism and tells the difference between gram negative and gram positive bacteria. Simple staining is unable to highlight the exact organism.


What are the uses of the gram stain technique scientifically speaking?

Gram stains are used to analyze bacteria and determine their characteristics. The most popular use of a gram stain is to determine the thickness of a cell wall in bacteria.


Why do you use iodine to stain a cell?

Iodine stains starch contained in cells. Iodine is also used to distinguish between Gram negative and Gram positive bacteria. The Gram stain contains iodine.

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