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Why is sterization?

Anonymous

∙ 16y ago

Updated: 9/17/2023

to kill microbes

Wiki User

∙ 16y ago

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Why you sterlize at 121 Celsius temp for 15 minutes in autoclave?

The sterilisation temperature is defined by the spores of the Geobacillus stearothermophilus. The spores have a D-value of 1,5 min @ 121°C. D-value means decimal reduction criteria, so it means, that after a treatment of 1,5 min (90 sec) at 121°C 90% of the bacillus spores have "died". For your process that means a 10x 90% reduction, a 10D concept. This means after 15 min @ 121°C 99,99999999% of the spores of G. stearothermophilus are "gone". Usual, the food industry demands commercial sterility, which is defined with a 12D concept. The regular sterilization requirement is usually 20min @121°C (or 5min @ 135°C). Where is your 15min@121°C sterization concept used? Cheers, Abraxas

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