Gram staining (or Gram's method) is an empirical method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative) based on the chemical and physical properties of their cell walls. It is a first step to determine the identity of a particular bacterial sample. Gram stains are performed on body fluid or biopsy when infection is suspected. It yields results much more quickly than culture, and is especially important when infection would make an important difference in the patient's treatment and prognosis.
It allows you to observe the morphology of the bacteria under a microscope. Identification can be established due to the shape of configuration of the bacteria. You should be aware though that bacteria can exhibit many forms and it is only the most predominate one you need to assess. Colonies can divide in one plane and form linear, chain like patterns, in two planes or at right angles forming tetards or can even divide randomly forming grape like clusters.
Gram staining is used to differentiate between those bacteria that have an outer membrane and those that do not. Gram positive bacteria do not have an outer membrane and will appear purple/violet when stained. Gram negative bacteria contain an outer membrane and will appear red/pink when stained
WELL the result's is provided in 10 min
the proceuder is not diffecult
not expenseve
very helpfull for destinguish between the bacteria
The gram staining procedure is important in medicine because it is a typical way to distinguish different types of bacteria. Gram stain-positive generally are staph or strep bacteria.
Crystal violet
No. It is a staining on the cell itself.
as a couterstain
This is known as a gram test.
Gram staining is highly valuable. It allows us to identify two widely different bacteria. Gram staining can tell you if the bacteria is pathogenic or if a penicillin pill can cure it. It tells us gram-positive bacteria, or gram-negative. Positive being easily combated bacteria and some even helpful, and gram-negative being primarily pathogenic.
Bacteria are those that are stained dark blue or violet by Gram Staining.
yes it is a special staining like other types....its importance is that one can identify the type of bacteria........
No. It is a staining on the cell itself.
Gram staining was devised by Hans Christian Gram of Denmark in the 1800s. (1853-1938)
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
It tells the microbiologist/clinician facts about the cell wall construction of the bacterium. This then indicates which antibiotics to prescribe if you are trying to get rid of a bacterial infection.
gram positive
as a couterstain
Gram Positive Rods
A basic dye used in gram staining is crystal violet.
no, it's eukaryotic. Gram staining is for bacteria.
This is known as a gram test.