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What is the purpose of sodium azide in the Bile Esculin test?
Sodium azide is included in the Bile Esculin test to inhibit the growth of Gram-negative organisms, which could interfere with the test results. Sodium azide helps to maintain a selective environment that favors the growth of group D streptococci, the target organisms for the test.
You were instructed to compare the growth on the NA and Columbia CNA agar plates. Would it have been better to compare the organisms on the CNA plate?
Yes, comparing the growth on the Columbia CNA agar plate would have been more informative as it contains colistin and nalidixic acid inhibitors to select for Gram-positive organisms. This allows for better differentiation and identification of Gram-positive bacteria based on their ability to grow or inhibit growth on this selective media.
If you observed growth of gram negative organism on the pea plate you inoculated does this negate the usefulness as a selective medium?
The growth of gram-negative organisms on the pea plate does not necessarily negate its usefulness as a selective medium, but it does raise questions about its selectivity. If the medium is designed to inhibit gram-negative growth, the presence of such organisms suggests it may not be effectively selective for gram-positive bacteria. However, if the medium allows for some gram-negative growth while still favoring gram-positive organisms, it can still be considered useful, albeit with limitations. Further testing would be needed to evaluate its selectivity and effectiveness in specific applications.
Which gram positive bacteria grows on macconkey and EMB?
Most gram positive bacteria do not grow on MacConkey agar or Eosin Methylene Blue (EMB) agar, as these are selective media designed to inhibit the growth of gram positive organisms. So, typically, you would not expect a gram positive bacteria to grow on these media.
What is positive organisms?
"Positive organisms" is a term that typically refers to microbes that have beneficial effects on their environment, such as by promoting health or growth in plants, animals, or humans. Examples include probiotic bacteria in the gut that aid in digestion or nitrogen-fixing bacteria in soil that enhance plant growth.
What is the purpose of sodium azide in the Bile Esculin test?
Sodium azide is included in the Bile Esculin test to inhibit the growth of Gram-negative organisms, which could interfere with the test results. Sodium azide helps to maintain a selective environment that favors the growth of group D streptococci, the target organisms for the test.
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Why PEA contains 0.25 percent phenylethyl alcohol because high concentration inhibits both gram negative and gram positive?
At higher amounts, phenylethyl alcohol would most likely also inhibit gram positive organisms because it would be able to break down their membrane permeability barrier, thus allowing influx of substances ordinarily blocked and leakage of large amounts of cellular potassium. This ultimately disrupts or halts DNA synthesis.
What chemical is produced by a living organism that kills or inhibits growth?
Antibiotics are chemicals produced by living organisms that kill or inhibit the growth of other organisms, particularly bacteria.
How does E. Coli inhibit growth as a gram positive?
Escherichia coli (E. coli) is actually a Gram-negative bacterium, not Gram-positive. Its cell wall structure, characterized by a thin peptidoglycan layer and an outer membrane containing lipopolysaccharides, is different from that of Gram-positive bacteria. Gram-negative bacteria like E. coli can inhibit the growth of Gram-positive bacteria through the production of bacteriocins, which are antimicrobial peptides that target and disrupt the membranes of competing bacteria. Additionally, E. coli can outcompete Gram-positive organisms for nutrients and space in their environment.
Do Organisms need proper temperature for growth and development?
Yes, organisms have an optimal temperature range for growth and development. Temperatures outside this range can slow down or inhibit these processes. Extreme temperatures can even be lethal to some organisms.
Do EMB plate dyes inhibit growth of Gram-positive or negative bac?
EMB plate dyes inhibit the growth of Gram-positive bacteria and promote the growth of Gram-negative bacteria. The dyes in EMB plates help differentiate between the two types of bacteria based on their ability to ferment lactose. Gram-negative bacteria that ferment lactose will produce dark colonies with a metallic green sheen.
What is the media selective for fungal organism?
Media selective for fungal organisms include Sabouraud agar, Potato Dextrose Agar (PDA), and Mycobiotic agar. These media contain ingredients that inhibit the growth of bacteria while promoting the growth of fungi.
What is the purpose for adding salt to mannitol salt agar?
High concentrations of salt inhibit the growth of non-halophilic organisms but allow for the growth of bacteria from the genus Staphylococci.
What is the primary regulator of growth?
Genetics is the primary factor in an organism's ability to grow, although availability of nutrients can inhibit an organisms size as well.
You were instructed to compare the growth on the NA and Columbia CNA agar plates. Would it have been better to compare the organisms on the CNA plate?
Yes, comparing the growth on the Columbia CNA agar plate would have been more informative as it contains colistin and nalidixic acid inhibitors to select for Gram-positive organisms. This allows for better differentiation and identification of Gram-positive bacteria based on their ability to grow or inhibit growth on this selective media.
If you observed growth of gram negative organism on the pea plate you inoculated does this negate the usefulness as a selective medium?
The growth of gram-negative organisms on the pea plate does not necessarily negate its usefulness as a selective medium, but it does raise questions about its selectivity. If the medium is designed to inhibit gram-negative growth, the presence of such organisms suggests it may not be effectively selective for gram-positive bacteria. However, if the medium allows for some gram-negative growth while still favoring gram-positive organisms, it can still be considered useful, albeit with limitations. Further testing would be needed to evaluate its selectivity and effectiveness in specific applications.
