Autoclaving is a method of sterilizing equipment by the use of pressurized high temperature steam. It may be used to sterilize surgical equipment for reuse or for sterilizing bottles prior to filling them with beer, soda pop, or other beverages.
use a pressure cooker
Autoclaving
by autoclaving at 121°C for 15 minutes
Autoclaves use high preassure and temperature to kill anything that might infect your media etc.
yes
it is unacceptable
Autoclaving is used to kill all micro-organisms that are in the culture. Autoclaving is usually carried out at 121 deg C with steam under 2 atmospheres of pressure. A clean culture free of all micro-organisms is essential as you only want the organism of intrest to grow.
http://wiki.answers.com/Q/How_are_the_material_prepared_for_autoclaving"
Autoclaving is more efficient than boiling because it has a hotter temperature. This is sure to clean the tools correctly by sterilizing and remove all germs.
autoclaving
Polycarbonate products are autoclavable. They must be thoroughly rinsed before autoclaving because detergent residues cause crazing and spotting. Autoclaving cycles should be limited to 20 minutes at 121°C. PC shows some loss of mechanical strength after repeated autoclaving and therefore may not function well under high-stress applications, such as centrifugation.
Prions are only destroyed by:• incineration• autoclaving in 1N NaOH