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They do not move up because the aren't soluble the solvent
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∙ 13y ago
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How can substances be separated?
gas-liquid, paper, or column chromatography are some examples
What colours could have no effect in the process of chromatography?
This question is not very precise, but I assume you mean which colours will not be separated by chromatography. It is not a property of the colour, but of the substance you are trying to split up. Chromatography only works if the substance is soluble in the liquid you are using to run the chromatogram. Thus some black fountain pen inks separate in water, but the ink from a ball point pen usually does not.
What are the advantages and limitations of column chromatography over thin layer chromatography?
TLC. The mobile phase is a liquid, the stationary phase is a solid. Useful for seperating and comparing mobility of solids and some liquids dissolved in the mobile phase by their affinities to the solid phase relative to the mobile phase. GLC. The mobile phase ia s gas, the stationary phase is a liquid on a solid support. same concept as TLC. useful for seperating gases by their affinities to the stationary phase...the mobility can then be compared to known compounds for possible identification.
What is the separation process which works because some colours are absorbed more easily by paper than others?
selective absorption
How can paper chromatography be used to separate pigments based on their chemical and physical properties?
It is an appropriate technique to use because it separates the pigments, so one can see which pigments are present, even if some pigments are normally hidden to the naked eye.
What are some possible alternatives for paper chromatography?
Coffee filter paper can be used. It does not produce results as good as chromatography paper, but it still works.
How can substances be separated?
gas-liquid, paper, or column chromatography are some examples
What colours could have no effect in the process of chromatography?
This question is not very precise, but I assume you mean which colours will not be separated by chromatography. It is not a property of the colour, but of the substance you are trying to split up. Chromatography only works if the substance is soluble in the liquid you are using to run the chromatogram. Thus some black fountain pen inks separate in water, but the ink from a ball point pen usually does not.
What is paper chromatography?
Paper chromatography is a technique used to separate a mixture into its component molecules. The molecules migrate, or move up the paper, at different rates because of differences in solubility, molecular mass, and hydrogen bonding with the paper.
Chromatography separates a mixture of?
Chromatography separates a mixture of pigments, usually in inks. You can separate colours in food and felt tips. The different solubilities of the different ink pigments, make some rise above others so you can see them clearly.
Uses for paper chromatography?
They are used in many scientific studies to identify unknown organic and inorganic compounds. They are also used in crime scene investigation, DNA and RNA sequencing, among others. Essentially, any solution can be separated through some form of chromatography.
What is a chromtography?
Hi is it got to do with Science? Its Chromatography .. Actually right now I'm studying Chromatography it's a really interesting unit. It is a way of seeing the other different colours that are mixed together to make that colour. As an experiment you have filter paper and because it has very small holes that you cannot see with a naked eye, liquids are most likely to be the only ones passing through. Anyway, anything that has to do with colour e.g food colouring, permanent marker etc... the filter paper will absorb the ink and will spread make sure the ink is about 2cm up your paper. You then dip the filter paper into a beaker of water - only about a cm full of water. After a few minutes the ink will naturally spread and you will be able to identify what other colours are used to create the colour of your chosen type of ink or whatever. They use Chromatography for like police investigations with DNA and that sort of stuff ...
What might be some limitations to paper Chromatography?
the questions take a longer time to be answered the questions take a longer time to be answered
What is meant by tailing a spot in Chromatography?
Chromatography is a method of analyzing the contents of a mixed substance. It is performed by dissolving the substance in a suitable solvent. A drop or spot of the resulting liquid is deposited near the edge of a piece of absorbent paper, such as blotting paper or special chromatography paper. The strip of paper with the spot at the bottom is then suspended over a solvent with the bottom edge of the paper in the solvent. As the solvent soaks upwards, the solvent carries some particles upwards and away from the spot. Different compounds within the mixture will travel at different rates and eventually the strip of paper will show different bands of colors, separating out and indicating some of the various components that were in the original substance. 'Trailing a spot' is a short term for this laboratory process.
What are the advantages and limitations of column chromatography over thin layer chromatography?
TLC. The mobile phase is a liquid, the stationary phase is a solid. Useful for seperating and comparing mobility of solids and some liquids dissolved in the mobile phase by their affinities to the solid phase relative to the mobile phase. GLC. The mobile phase ia s gas, the stationary phase is a liquid on a solid support. same concept as TLC. useful for seperating gases by their affinities to the stationary phase...the mobility can then be compared to known compounds for possible identification.
How do you separate blue dye and red dye?
Chromatography-This is when you put dye on a piece of special paper and put it in some water. When the water is soak up the dye will separate.
What is a suitable solvent for paper chromatography?
In paper chromatography n-butanol:acetic acid:water mixture in the ratio 4:1:1 is used as a solvent whereas in case of thin layer chromatography(TLC) petroleum ether and acetone in the ratio 9:1 is used as a solvent. Also, in some cases, DCM(dichloromethane is used as a solvent.
