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Thioglycollate broth is an enriched medium used to determine the oxygen requirements of microorganisms. The diffusion of oxygen from the top of the broth provides a range of concentrations.
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Does b.subtilis facultative anaerobes in thioglycollate broth?
No
How can you tell if thioglycollate medium is prepared correctly?
Thioglycollate medium is correctly prepared when a red/brown color appears at the top of the broth. This is beacuse of the Reazurin which is reactive to oxygen.
What type of media is used to demonstrate oxygen requirements of microbes?
thioglycollate
What does a blue or pink color in thioglycollate mean?
If its Fluid thioglycollate media - a red colour indicates thatg the media has been oxygenated - which is not desirable. The colour change is due to a chemical called resazurin in the media.
Can a strict aerobe be distinguished from an anaerobe in thioglycollate broth?
Yes. Thioglycollate is a reducing medium that can establish a gradiation in oxygen content. Oxygen is highest at the top of the tube and absent in the deeper regions.Source: "Foundations in Microbiology" by Kathleen Park Talaro.
The properties and purposes of thioglycollate medium?
A thioglycollate medium is used for culturing anaerobic bacteria.
Does b.subtilis facultative anaerobes in thioglycollate broth?
No
How can you tell if thioglycollate medium is prepared correctly?
Thioglycollate medium is correctly prepared when a red/brown color appears at the top of the broth. This is beacuse of the Reazurin which is reactive to oxygen.
What is the function of thiogllycollic acid in thioglycollate?
It removes trapped oxygen from the medium
What type of media is used to demonstrate oxygen requirements of microbes?
thioglycollate
What does Thioglycollate contribute to culture media?
Look for this information in the link below.
What does a blue or pink color in thioglycollate mean?
If its Fluid thioglycollate media - a red colour indicates thatg the media has been oxygenated - which is not desirable. The colour change is due to a chemical called resazurin in the media.
Why will obligate anaerobes grow in thioglycollate?
Obligate anaerobes do not need or use oxygen as a nutrient. In fact it is a toxic substance to them which either kills or inhibits their growth. Thioglycollate allows obligate anaerobes to grow because it consists of sodium thioglycollate, thioglycollic acid and L-crystine which reduce oxygen to water. This creates an anaerobic environment allowing obligate anaerobes to grow.
Can a strict aerobe be distinguished from an anaerobe in thioglycollate broth?
Yes. Thioglycollate is a reducing medium that can establish a gradiation in oxygen content. Oxygen is highest at the top of the tube and absent in the deeper regions.Source: "Foundations in Microbiology" by Kathleen Park Talaro.
How does the thioglycollate model of peritonitis work?
In the thyoglycollate model of peritonitis, researchers inject thyoglycollate broth into the peritoneal cavities of laboratory animals to induce peritonitis. Then they test their materials to see if they are effective in curing peritonitis.
Why can media such as brain-heart infusion and thioglycollate be used for the cultivation of anaerobes?
Anaerobes cannot be cultivated in the presence of atmospheric oxygen.
Why you use Fluid Thioglycollate medium for bacterial contamination checking?
Fluid Thioglycollate is a medium used to grow and observe all manner of aerotolerance in bacteria. Therefore if you were to place a sample in it to see if it contained bacteria or not, given the correct temperature needed, the bacteria would be able to be observed fairly quickly as they would grow exponentially in this nutrient rich environment.
