The bacteria would be grounded up with the beef and it would be spread throughout the meat, making it ideal for the media to have bacterial growth throughout. Unlike the steak or roast where it would only have bacterial growth on the surface.
Nutrients and oxygen
Refers to the bacteria suspended on the petri dish or growth medium.
stool culture
surface area = greater availability to nutrients
Agar, which is a polysaccharide polymer, is a solidifying agent for culture medium. It also provides a solid surface for bacterial growth.
Incubation time, incubation temperature, and the medium used to grow the bacteria.
When lipids are added to an agar-solidified culture medium and are cultured with lipolytic bacteria, the surrounding medium becomes acidic due to the release of fatty acids. By adding a pH indicator (such as Spirit Blue which has a lavender color), it is possible to detect lipolysis by a clear area surrounding the bacterial growth. A clear area around bacterial growth indicates a positive test. In the absense of lipolysis, the medium retains its lavender color and is a negative reaction.
The purpose of both is to inhibit bacterial growth. The media is used for growing fungi.
because the growth of the unknown bacterial colony will create a pattern when it is grown that is characteristic and can help identify the unknown organism
Ampicillin is an antibiotic that is usually used as a reporter gene in cloning. A plasmid containing the ampicillin resistance gene (as well as another target gene within the plasmid) is introduced into the bacterial host. If the bacterium has taken up the plasmid and is expressing the plasmid, it will be resistant to ampicillin. LB is used as a growth medium and ampicillin to verify the plasmid is within the bactrium. No growth means no plasmid in the bacterial host...
Wet ground will clog the tiller. Better done to dry to medium moist soil.
• Basal Medium - contains no growth factors cytokines, or supplements• Supplement Kit - growth factors, cytokines, and supplements• Medium Kit - basal medium and Supplement Kit packaged together