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PCR products produce million copies of your gene of interest. After PCR, we usually resolve them on the agarose gel to visualize the amplified DNA using EtBr stain under UV. The main purpose is, it make sure your gene is really amplified and the length it run is corresponding to the right size of your gene of interest and purify it from other template DNA and other unspecifically amplified DNA products by extracting from the gel.
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What is used to sort DNA into different lenghts?
Gel Electrophoresis
What does resolve mean in gel electrophoresis?
The ability to separate compounds sufficiently by their electric charge.
What kind of controls are run in the gel electrophoresis experiment?
what kind of controls are run in the expirement? why are they important? could others be used? (PCR lab)
What is gel electrophoroses?
The migration of DNA/Protein on the gel (agarose/polyacrylamide) by the influence of electric charge is called gel electrophoresis. It is used to resolve the biomolecules according to their size(mainly) and shape(for proteins)
what is the gel in Gel Electrophoresis?
For larger molecules like proteins we use polyacrylamide gel electrophoresis (PAGE). For smaller pieces like DNA we use agarose gel electrophoresis
What has to happen before you can run DNA through gel electrophoresis?
Pcr
What is used to sort DNA into different lenghts?
Gel Electrophoresis
What does resolve mean in gel electrophoresis?
The ability to separate compounds sufficiently by their electric charge.
How do you reduce multiple band in agarose gel electrophoresis?
I think you should increase the annealing temperature and also reduce the PCR cycles.
What kind of controls are run in the gel electrophoresis experiment?
what kind of controls are run in the expirement? why are they important? could others be used? (PCR lab)
What does the accuracy of the fragment sizes tell you about the resolving ability of agarose-gel electrophoresis?
The larger fragements will not be very accurate because they cannot resolve in high consentrations of the agarose in the gel. The percent of agarose in the gel affects the ability to resolve larger fragements of DNA
What is gel electrophoroses?
The migration of DNA/Protein on the gel (agarose/polyacrylamide) by the influence of electric charge is called gel electrophoresis. It is used to resolve the biomolecules according to their size(mainly) and shape(for proteins)
what is the gel in Gel Electrophoresis?
For larger molecules like proteins we use polyacrylamide gel electrophoresis (PAGE). For smaller pieces like DNA we use agarose gel electrophoresis
A scientist uses material in her lab to separate dna fragments by size .what process is she using?
PCR or polymerase chain reaction. :: Apex
Can a gel electrophoresis be performed both horizonnally and vertically?
yes for example 2D gel electrophoresis
A technique that can be used to compare the DNA of two or more plants is?
Gel electrophoresis
What is a common name for gel electrophoresis?
gel
