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control of copy number in plasmids

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Q: Why do some plasmids have high copy number while others have low copy number?
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Is plasmid that can replicate in Ecoli and Pseudomonas a high copy number plasmid?

Many Pseudomonas plasmids are transmissible to E. coli, but the copy number is plasmid-dependent. Some plasmids are of low-copy number whereas some are high-copy number, and we should also consider copy-number-incompatibility. It could not be said that plasmids which can replicate in E.coli and Pseudomonas are of high copy number.


What is plasmid copy number?

The copy number reflects the average number of copies of a certain plasmid inside a host cell. The higher the copy number, the more efficient the plasmid is at replicating itself. Researchers using plasmids as vectors usually choose high copy number plasmids as their vectors since you can get a large number of plasmids from relatively fewer cells in less time.


How many plasmids in a bacterial cell?

The number of plasmid in a bacterial cell,which is otherwise known as Plasmid Copy Number(PCN), may vary from a mere one to a whopping thousand


Why plasmid are circular?

Plasmids are genetic structures in a cell that can replicate independently of the chromosomes. They are circular because linear DNA requires a way to copy its ends.


What is the difference between single copy control and multi-copy control DNA replication?

•single-copy replication control -A control system in which there is only one copy of a replicon per unit bacterium. -The bacterial chromosome and some plasmids have this type of regulation. •multicopy replication control -Occurs when the control system allows the plasmid to exist in more than one copy per individual bacterial cell. Contribution by: mahdi


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