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what are the various methods of the Hb estimation???/kindly tell me the senstivity of the methods. i know only one method sahli hemoglobinometer(acid hematin) method.

I have enumerated few

Sahli - Adam's Method

Dare's method

Haden's method

Wintrobe's method

Haldane's method

Talliquist method

Gasometric method

Spectrophotometric method

Automated & non-automated hemolglobinometry

Other methods

alkaline hematin method

specific gravity

comparator method

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13y ago
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10y ago

In this method, the ferrous form of Hb is converted to ferric form in presence of potassium ferricyanide, converted to met-haemoglobin which is then converted to cyanmethemoglobin in presence of potassium cyanide.

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11y ago

According to me Sahli' Methods is the best method for estimation of HB

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Q: What is the principle of hemoglobin estimation using cyanmethemoglobin method?
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Advantages and disadvantages using cyanmethemoglobin method?

The Cyanmethemoglobin method is a commonly used laboratory procedure for the estimation of hemoglobin in blood. This method has several advantages and disadvantages. Advantages: Accuracy: The Cyanmethemoglobin method is considered very reliable and is often used as a reference method. Stability: The Cyanmethemoglobin solution is stable, and readings can be taken at a later time without significant alteration. Interference: It is less susceptible to turbidity and lipemia interference as compared to other methods. Disadvantages: Toxicity: The reagent used, Drabkin's solution, contains potassium cyanide, which is toxic and needs to be handled with care. Time: The reaction time to obtain cyanmethemoglobin is relatively longer compared to other methods. Interference: The method can overestimate hemoglobin if a large number of white cells, such as in leukemias, are present. In conclusion, while the Cyanmethemoglobin method has its advantages, including accuracy and stability, it also has some disadvantages such as toxicity of the reagent used and potential interference in certain conditions. It's crucial to carefully consider these factors before deciding on a method for hemoglobin estimation.


Which form of hemoglobin cannot be measured using the cyanmethemoglobin method?

sulphaemoglobin shb


What is the principle of the cyanmethemoglobin method?

Colorimetric method where blood is mixed with solution containing potassium ferricyanide and potassium cyanide. Potassium ferricyanide oxidizes iron to form methemoglobin and potassium cyanide then combines with methemoglobin to form cyanmethemoglobin.


Disadvantages using cyanmethemoglobin method?

three disadvantages cyanmethemoglobin


Which are the Methods for hemoglobin estimation?

Hemoglobin estimation is the measuring of hemoglobin levels that are broken into three categories. Visual methods are Sahil, Dares, Hadens, Wintrobes, Haldanes, Tallquists method. Second to consider are the gasometric method, single to the Van Slyke method. Lastly the spectrophotometric method oxyhemoglo, and the cyanmethemoblobin method.


What is tallquist method testing for?

It is a method of hemoglobin estimation in blood. The process involves absorbing a drop of blood on a standard paper and comparing it with a standard specimen.


What is the significance of the development of the cyanmethemoglobin method?

ewan ko sayo,assignment mo yan eh...


What is front end estimation?

Front end estimation- An estimation method in which the front digits are added or subtracted


Is lowry's protein estimation an invasive method?

No. It is a non-invasive method


What is a method for math in a estimation?

round or rounding


What is an invasive method of protein estimation?

gand mara


Hb estimation by drabkin's method?

Procedure to estimate haemoglobin in blood by cyanomethaemoglobin method