what are the various methods of the Hb estimation???/kindly tell me the senstivity of the methods. i know only one method sahli hemoglobinometer(acid hematin) method.
I have enumerated few
Sahli - Adam's Method
Dare's method
Haden's method
Wintrobe's method
Haldane's method
Talliquist method
Gasometric method
Spectrophotometric method
Automated & non-automated hemolglobinometry
Other methods
alkaline hematin method
specific gravity
comparator method
In this method, the ferrous form of Hb is converted to ferric form in presence of potassium ferricyanide, converted to met-haemoglobin which is then converted to cyanmethemoglobin in presence of potassium cyanide.
According to me Sahli' Methods is the best method for estimation of HB
The Cyanmethemoglobin method is a commonly used laboratory procedure for the estimation of hemoglobin in blood. This method has several advantages and disadvantages. Advantages: Accuracy: The Cyanmethemoglobin method is considered very reliable and is often used as a reference method. Stability: The Cyanmethemoglobin solution is stable, and readings can be taken at a later time without significant alteration. Interference: It is less susceptible to turbidity and lipemia interference as compared to other methods. Disadvantages: Toxicity: The reagent used, Drabkin's solution, contains potassium cyanide, which is toxic and needs to be handled with care. Time: The reaction time to obtain cyanmethemoglobin is relatively longer compared to other methods. Interference: The method can overestimate hemoglobin if a large number of white cells, such as in leukemias, are present. In conclusion, while the Cyanmethemoglobin method has its advantages, including accuracy and stability, it also has some disadvantages such as toxicity of the reagent used and potential interference in certain conditions. It's crucial to carefully consider these factors before deciding on a method for hemoglobin estimation.
sulphaemoglobin shb
Colorimetric method where blood is mixed with solution containing potassium ferricyanide and potassium cyanide. Potassium ferricyanide oxidizes iron to form methemoglobin and potassium cyanide then combines with methemoglobin to form cyanmethemoglobin.
three disadvantages cyanmethemoglobin
Hemoglobin estimation is the measuring of hemoglobin levels that are broken into three categories. Visual methods are Sahil, Dares, Hadens, Wintrobes, Haldanes, Tallquists method. Second to consider are the gasometric method, single to the Van Slyke method. Lastly the spectrophotometric method oxyhemoglo, and the cyanmethemoblobin method.
It is a method of hemoglobin estimation in blood. The process involves absorbing a drop of blood on a standard paper and comparing it with a standard specimen.
ewan ko sayo,assignment mo yan eh...
Front end estimation- An estimation method in which the front digits are added or subtracted
No. It is a non-invasive method
round or rounding
gand mara
Procedure to estimate haemoglobin in blood by cyanomethaemoglobin method