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First of all the function of the gel electrophoresis is to separate lengths of DNA samples by using different charges. The function of the gel is to allow the DNA fragments to travel downward so we can differentiate between the lengths. The gel will allow the smaller segments to travel faster. If the gel was made thick the segments would travel slower.
Wiki User
∙ 13y ago
Wiki User
∙ 12y agoHi I am going to make note of the source for the sake of convineance: Conoley C, (2002), Chemistry, London: HarperCollinsPublishers ISBN: 0-00-713597-1 (page no:418).
Positiveley charged ions migrate to the cathode (-ve) and negativeley charged ions to the anode (+ve). When the pH is that at which zwitterions* are fromed (the isoelectric point) with equal positive and negative charges the zwitter ions cannot move in either direction. so, the pH value must be carefully chosen before the mixture of amino acids can be separated xan be separated and analysed successfully.
*A zwitterion is formed when an amino acid undergoes internal neutrilisation, by this I mean the the H+ ion on the COOH group goes to the NH2 group (the amine group) to form an NH3 group thus for one is positive (NH3+) and one is negative (COO-) and they are therefore neutral.
NB: Gel electrophoresis works on the separation of proteins (and therefore DNA aswell) don't forget that amino acids are what make up proteins so don't at any point think that the reference to amino aicds in this context is abstract.
Hope this helps Nick C
Wiki User
∙ 13y agoto act as a sieve, slowing down the movement of larger particles as they migrate through the gel.
Wiki User
∙ 14y agoThe wells are a starting point for the DNA. The distance between where the fragments ended up and the well is the total distance the fragments moved.
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What are the pros and cons of gel electrophoresis?
Pros: The detection of DNA, RNA and proteins can be done using gel electrophoresis. Gel electrophoresis does not require a large amount of starting material. Cons: difficult to extract samples for further analysis. Harmful materials.
Are restriction enzymes used in gel electrophoresis?
For DNA gel electrophoresis, yes. Once the DNA is cut up into different-sized fragments, they can be electrophoresed to separate bands.
How does gel electrophoresis is used to make a DNA fingerprint?
Gel electrophoresis separates DNA fragment on the basis of their size. In DNA fingerprinting or DNA typing given sample is cut up with restriction enzymes and run through electrophoresis and results are analyzed to check for DNA polymorphism between the given sample and a sample form suspect. In nutshell gel electrophoresis is boon for the people in forensics.
What is the conclusion on the application of gel electrophoresis?
One of the Conclusion of electrophoresis is Visualization of the DNA size. Second is Sequencing the length of DNA of the body.
What is th blue dye added to the sample before the electrophoresis is performed?
In biochemistry labs, the traditional answer for a protein gel (polacrylamide gel electrophoresis) is bromphenol blue. For a DNA gel (agarose gel electrophoresis), traditionally the same dark blue dye bromphenol blue was combined with the lighter, slower migrating blue dye xylene cyanol. Oftentimes nowwe only use the bromphenol blue, or even substitute for it with Orange G, which is a UV-transparent dye that more easily enables the visualization of smaller molecular weight nucleic acids that migrate in the same region.
What is the function of the gel electrophoresis?
Gel electrophoresis is an analytical method used to separate DNA, RNA or proteins based on size
What is used to sort DNA into different lenghts?
Gel Electrophoresis
What is the function of buffer in the gel electrophoresis?
TBE buffer in gel electrophoresis is used to maintain pH of te solution and prevents the denaturation of smale fragments of DNA.
what is the gel in Gel Electrophoresis?
For larger molecules like proteins we use polyacrylamide gel electrophoresis (PAGE). For smaller pieces like DNA we use agarose gel electrophoresis
Can a gel electrophoresis be performed both horizonnally and vertically?
yes for example 2D gel electrophoresis
A technique that can be used to compare the DNA of two or more plants is?
Gel electrophoresis
What is a common name for gel electrophoresis?
gel
Where can one learn more on gel electrophoresis?
To learn more about gel electrophoresis, one can Google it. There is also a whole Wikipedia article dedicated to gel electrophoresis, and it happens to be quite informative.
What has the author A J Houtsmuller written?
A. J. Houtsmuller has written: 'Agarose-gel-electrophoresis of lipoproteins' -- subject(s): Blood protein electrophoresis, Electrophoresis, Gel electrophoresis, Lipoproteins
Process that restriction fragments of DNA are separated from each other by the use of electricity?
The process is referred to as gel electrophoresis. This is an analytical process where DNA fragments can be separated based on size within a gel under the influence of an electric field
Can gel electrophoresis be used to determine the purity of an enzyme?
Yes it can be done if you use Poly-Acrylamide Gel Electrophoresis.
Difference between vertical and horizontal gel electrophoresis?
Horizantal gel electrophoresis is generally used for RNA/DNA based studies, while vertical gel electrophoresis is used for protein based studies.
