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What is a homogenate?

Updated: 4/28/2022
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15y ago

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Homogeneous means any property of that substance would be the same everywhere and in any direction. For example density. That would be the same at each and every location of the substance. Say speed of light within the medium. That would be the same in all direction. Hence such a substance is known to be a Homogeneous one

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Rick Satterfield

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1y ago
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15y ago

Cells are broken up by a homogeniser (blender). This releases the organelles from the cell. The resultant fluid is known as the homogenate, which is then filtered to remove any complete cells and large pieces of debris

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11y ago

Homogeneous means any property of that substance would be the same everywhere and in any direction. For example density. That would be the same at each and every location of the substance. Say speed of light within the medium. That would be the same in all direction. Hence such a substance is known to be a Homogeneous one

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15y ago

composed of parts or elements that are all of the same kind

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Q: What is a homogenate?
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What apparatus or tools can be used in homogenizing sample product?

Many tools are available for the homogenization of samples. These homogenizers can be generally grouped into those that grind, shear, beat, and shock, though many disruption processes rely on more than one type of force. When developing a disruption scheme, it is critical to define the characteristics of the homogenate and then select the tools that will help to meet the objectives. The examination of methods used to homogenize samples has shown that effectiveness is directly related to the nature of the sample. Samples that start with small particles, such as bacterial cultures, are most effectively disrupted by ultrasonication, but that same method is the poorest for solid muscle. In such cases, samples must be first disaggregated into smaller particles prior to processing. Methods which rely on a single processing step, such as with the high throughput homogenizers, can yield very good sample disruption, but they do not match two-step processes that breakdown samples in a series of steps. The need to process large numbers of samples requires a trade-off with level of effective homogenization. Another, pp bags can be used in simple homogenization process. David W. Burden, Ph.D.


How do you estimate protein content in osyter mushroom?

ISOLATION OF MUSHROOM PROTEINSANDESTIMATION OF TOTAL PROTEIN BY LOWRY'S METHOD.Reagents Required1. BSA stock solution (1mg/ml),2. Analytical reagents:a)50 ml of 2% sodium carbonate (2g in 100 ml of d)10 ml of 1.56% copper sulphate solution mixed with 10 ml of 2.37% sodium potassium tartarate solution.Prepare analytical reagents by mixing 2 ml of (b) with 100 ml of (a)3. Folin - Ciocalteau reagent solution (1N) Dilute commercial reagent (2N) with an equal volume of water on the day of use (2 ml of commercial reagent + 2 ml distilled water)ProcedureA. Isolation of proteins from Mushrooms2 grams of mushroom were weighed properly and minced by using 10 ml phosphate buffer supplemented with 2 % SDS (sodium dodecyl sulfate) by using pestle and mortar.The homogenate was filtered through double layered cheese cloth.The filtrate was subjected to ammonium sulphate saltprecipitation method at 65% saturation.The proteins were pelleted by centrifugation and then dried.C. Estimation of total proteins by Lowry method:1. Different dilutions of BSA solutions are prepared by mixing stock BSA solution (1 mg/ ml) and water in the test tube as given in the table. The final volume in each of the test tubes is 2 ml.2. For these protein solutions add 2 ml of alkaline copper sulphate reagent (analytical reagent). Mix the solutions well.3. This solution is incubated at room temperature for 10 mins.4. Then add 0.2 ml of reagent Folin Ciocalteau (FC) solution (reagent solutions) to each tube and incubate for30 min at room temperature.5. By using blank, Zero the colorimeter and take the optical density (measure the absorbance) at 660nm.5. Plot the absorbance against protein concentration to get a standard calibration curve.6. Check the absorbance of unknown samples and determine the concentration of the unknown sample using the standard curve plotted above.Calculation:Standard calculation:From standard solution 1mg is 1ml, it indicates 1000 µg in 1 ml of sample, hence 0.2ml contains 200 µg similarly 0.4 ml contains 400 µg of proteins and so on.Determination of unknown concentration:From standard graph 0.4 corresponds to X µg of proteinsThat indicates 0.5 ml of sample contains X µg of proteinsHence 100ml of unknown sample contains (X x 100) / 0.5 =----- µg of proteinRESULT:The 100ml of present in mushroom sample isolated contains----- µg or------ mg of protein


What are some words end with the suffix ate?

Some words with the suffix 'ate' are: abate abbreviate abdicate ablate abnegate abominate abrogate accelerate accentuate acclimate accommodate acculturate accumulate accurate acerbate acetate acetylate acidulate acrylate activate actuate aculeate acuminate acylate adequate adjudicate administrate adnate adsorbate adulate adulterate adumbrate advocate aerate aestivate affectionate affiliate affricate agate agglomerate agglutinate aggravate aggregate agitate agnate alate alginate alienate aliterate alkylate alleviate alliterate allocate altercate alternate aluminate aluminosilicate alveolate amalgamate ambulate ameliorate ammoniate amputate angulate animate annihilate annotate annulate annunciate antedate antepenultimate anticipate antiquate apostate apostolate appellate appreciate approbate appropriate approximate arbitrate archiepiscopate arcuate areolate arillate arpeggiate arrogate arsenate articulate ascorbate aspartate asphyxiate aspirate assassinate asseverate assimilate associate ate attenuate aureate auriculate auscultate authenticate automate autorotate aviate baccalaureate baccate backdate baldpate barbiturate bate bedmate bedplate benzoate berate bicarbonate bichromate biflagellate bifurcate billingsgate bilobate binate binucleate bipinnate birthdate birthrate bistate bisulfate bitartrate bivariate boilerplate bombinate bookplate borate borosilicate brachiate bracteate breastplate brecciate bromate brominate bullate bunkmate butylate butyrate cachinnate calculate calibrate caliphate calumniate campanulate camphorate candidate cantillate capacitate capitate capitulate captivate carbamate carbohydrate carbonate carboxylate cardinalate carinate carpellate caseate casemate castigate castrate cate catenate caudate celebrate celibate cellmate cerebrate certificate chalybeate cheapskate checkmate chelate chlorate chlorinate chocolate chordate chromate ciliate cingulate circinate circulate circumambulate circumnavigate circumstantiate circumvallate citrate 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modulate molybdate motivate mucronate multinucleate multivariate muriate muricate mutate mutilate nameplate narrate nauseate navigate necessitate negate negotiate neonate nictate nictitate nitrate nominate notate novitiate nucleate numerate nutate obcordate obdurate obfuscate objurgate oblanceolate oblate obligate obliterate obnubilate obovate obstinate obturate obviate odonate officiate oleate operate operculate opiate orate orchestrate ordinate organophosphate orientate originate ornate orthophosphate oscillate osculate outdate outdebate outstate ovate overate overcompensate overcomplicate overdecorate overeducate overelaborate overestimate overmedicate overpopulate overrate oversaturate overstate overstimulate ovulate oxalate oxidate oxygenate paginate palate palatinate palliate palmate palmitate palpate palpitate paniculate pantothenate papillate participate particulate passionate passivate pastorate pate patriarchate patriciate pectate pectinate peculate pedicellate pedunculate 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schoolmate scintillate sedate segregate selenate semiliterate semiprivate senate sensate separate sequestrate seriate serrate shipmate shogunate sibilate sigmate silicate simulate situate skate slate soleplate solvate somnambulate sonicate sophisticate spate spathulate spatulate speciate spectate speculate sporulate squamate stablemate stagnate stalemate staminate state stearate stellate stimulate stipulate stomate strangulate striate stridulate stylobate subjugate sublate sublimate subordinate subrogate substantiate substrate subtemperate subulate succinate suffocate sulcate sulfate sulfonate sulphate sultanate summate superannuate superordinate superphosphate supersaturate superstate supinate supplicate suppurate suricate surrogate syllabicate syncopate syndicate tabulate tailgate tannate tartrate teammate temperate template terephthalate tergiversate terminate ternate tessellate testate thermoregulate thiocyanate thiosulfate tinplate titanate titillate titivate titrate tolerate tollgate tractate transilluminate translate transliterate translocate transmigrate transubstantiate transudate triacetate triangulate tribunate trifoliate trifurcate triphosphate triplicate tristate triturate triumvirate truncate tuberculate tungstate tunicate turbinate ulcerate ultimate ululate umbellate umbilicate unaffectionate uncinate uncrate uncreate underestimate undergraduate underrate understate undulate unfortunate ungulate uninitiate uninucleate univariate unregenerate update upstate urinate urochordate urticate vacate vaccinate vacillate vacuolate validate valuate variate variegate vaticinate vegetate venerate ventilate vermiculate vertebrate verticillate vesiculate vibrate vicariate vindicate violate vitiate vituperate vizierate vociferate vulgate vulvate workmate xanthate


What are 10 letter words that end in E?

confinecompeteconsumeconfusediseaserestaterespitedeclinedivorcediversedefenseenforceinflameinclinemistakerestaterepleteremorsereclinesqueeze