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DNA hydrolysis is the breaking of DNA through the addition of water. It can be done in several fashions; enzymatic (exonucleases)or chemicals (acid).
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What is a linker DNA?
Linker DNA links adjacent nucleosome core particles to each other. They then resemble beads on a string. These linker DNAs are readily removed through exonuclease hydrolysis.
What is the chemical reaction for the hydrolysis of DNA using Sulfuric acid?
Complete hydrolysis of chromosomal nucleic acids gave inorganic phosphate, 2-deoxyribose and four different heterocyclic bases
Purpose of pepsin in DNA extract?
Pepsin is an enzyme that catalyzes the breakdown of proteins into polypeptides and amino acids through hydrolysis. In DNA extraction, this proteolytic processing is essential in dissolving the cellular and histone proteins that bind the DNA strands.
What does complete hydrolysis of DNA yield?
kung taga clsu ka, wag ka dito umasa, haha
Does hydrolysis use ATP?
The hydrolysis of ATP is ADP
Use of NaCl in DNA Hydrolysis?
Salt is used to separate DNA.
DNA is more resistant to hydrolysis than RNA what feature of DNA is responsible for this characteristic?
bonds
What is a linker DNA?
Linker DNA links adjacent nucleosome core particles to each other. They then resemble beads on a string. These linker DNAs are readily removed through exonuclease hydrolysis.
What is is a linker?
The DNA found between nucleosomes on chromatin; since it is not complexed to proteins as strongly as other forms of dna, it is accessible to exonuclease hydrolysis.
Principle involved in the test for phosphate of DNA?
hydrolysis of pyrophosphate to phosphate forming yellow ppt.
What is the chemical reaction for the hydrolysis of DNA using Sulfuric acid?
Complete hydrolysis of chromosomal nucleic acids gave inorganic phosphate, 2-deoxyribose and four different heterocyclic bases
What do biologists use to cut DNA into smaller fragments?
A restriction enzyme (or restriction endonuclease) is an enzyme that cuts double-stranded or single stranded DNA at specific recognition nucleotide sequences called restriction sites.
Purpose of pepsin in DNA extract?
Pepsin is an enzyme that catalyzes the breakdown of proteins into polypeptides and amino acids through hydrolysis. In DNA extraction, this proteolytic processing is essential in dissolving the cellular and histone proteins that bind the DNA strands.
Role of te in dna extraction?
TE stands for Tris and EDTA. The Tris buffers the water to prevent acid hydrolysis of the DNA/RNA. The EDTA chelates divalent cations that can assist in the degradation of RNA.
What is the difference between acidic and alkaline hydrolysis?
Acidic hydrolysis: hydrolysis in an acid solution (pH under 7) Basic hydrolysis: hydrolysis in a basic solution (pH above 7)
What enzyme breaks the bonds between the complementary parent strands?
Hydrolysis(break) of a phosphodiester bond, separates two strands of DNA Strand breakage by the DNA untwisting enzyme results in covalent attachment of the enzyme to DNA
Is a peptide bond hydrolysis or dehydrates?
hydrolysis
